实时荧光定量PCR检测转基因玉米NK603品系特异片段的测量不确定度  

Uncertainty in Measuring Flanking Fragments of Genetically Modified Maize,Event NK603,by Quantitative Real Time PCR

作　　者：宋君[1] 雷绍荣[1] 郭灵安[1] 王东[1] 叶先林[1] 刘文娟[1] 尹全[1] 张富丽[1] 常丽娟[1] 

机构地区：[1]四川省农业科学院分析测试中心,四川成都610066

出　　处：《西南农业学报》2013年第5期1769-1773,共5页Southwest China Journal of Agricultural Sciences

摘　　要：应用转基因玉米NK603品系特异实时定量PCR方法,测定含量为2%的转基因玉米NK603样品中旁侧片段(品系特异片段)的含量,并从扩增反应、数据处理以及微量移液器等不确定度来源,评定了测量的不确定度。结果表明,u A=0.0008,u B=0.001,u C=0.001,U=0.002,测量结果为1.9%±0.002。NK603品系特异实时定量PCR方法检测结果的主要不确定度来自检验过程中的随机效应。The detection method of genetically modified maize,Event NK603,was used to measure the flanking fragment in samples and we evaluated the uncertainty of detection according to the sources of uncertainty,the PCR system,data analysis and micropipette.The results showed that A type of uncertainty was 0.0008,B Type of uncertainty was 0.001,combined standard uncertainty was 0.001,expanded uncertainty（U） was 0.002 and the finally measured result was 1.9 ％ ± 0.002 ％.Thus the main uncertainty of the result measured by this method came from the randomizing effect in the experimental process.

关 键 词：转基因玉米NK603 旁侧片段含量 不确定度 

分 类 号：S513[农业科学—作物学]