DEFB135基因多态性及表达与家兔NSDD的相关性研究  

Polymorphisms and Expression of DEFB135 Gene in Non-specific Digestive Disorders of Rabbit

作　　者：张娟丽[1] 张文秀[1] 彭瑾[1] 张龚炜[1] 杨宇[1] 王杰[1] 赖松家[1] 

机构地区：[1]四川农业大学动物遗传育种研究所,四川成都611130

出　　处：《西南农业学报》2013年第5期2084-2087,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家现代农业技术体系项目(CARS-44-A-2);四川省"十二五"科技支撑项目:天府黑兔新品种选育及配套技术研究与示范(2011NZ0099-4)

摘　　要：基于病例对照研究的试验设计,本试验对β-防御素135基因（β-defensin 135,DEFB135）非编码区和编码区进行单核酸多态性（SNPs）检测,在5’非编码区（5’-UTR）检测到2个SNPs（c.-47 T＞A,c.-40C ＞A）,3’-UTR区检测到1个SNP（c.＊42C＞G）,它们处于完全连锁状态.选取c.-40 C ＞A位点进行基因分型,研究DEFB135基因多态性与家兔非特异性消化道疾病易感性间的相关性.结果发现健康组和肠炎组中等位基因A的频率分别为30％和33％,差异不显著（P＞0.05）;OR（odds ratio）值为1.11（95％的置信区间：0.849～1.456,P=0.44）.低纤维诱导试验中,DEFB135基因在健康组,低轻微组和严重组中的表达量分别为0.0956±0.0273,0.0492±0.0228和0.0475±0.01703,差异均不显著P＞0.05.这些结果说明DEFB135基因可能与家兔NSDD不相关.In this case-control study,the polymorphism and expression level β-defensin 135 （DEFB135） were investigated.Primers were designed to amplify untranslated region （5＇-UTR,3＇-UTR）,all two exons.Two single nucleotide polymorphisms （SNPs,c.-47 T ＞ A,c.-40 C ＞ A） in 5＇-UTR and one SNP （c.＊ 42 C ＞ G） in 3＇-UTR were detected with 100 ％ linkage.The c.-40 C ＞ A SNP was randomly selected and genotyped to analyze the association with non-specific digestive disorder （NSDD）.The frequencies of A allele in control and case groups were 30 ％ and 33 ％ with no significant difference （P ＞ 0.05）,respectively.The odds ratio was 1.11 （95 ％ confidence interval,0.849-1.456,P =0.44）.The gene expression level of DEFB135 in three groups were 0.0956 ± 0.0273 （health individuals）,0.0492 ± 0.0228 （mild NSDD individuals） and 0.0475 ± 0.01703 （severe NSDD individuals） with no significant difference （P ＞ 0.05）.In conclusion,the DEFB135 gene may be not significantly associated with NSDD in rabbit.

关 键 词：DEFB135 病例对照研究 SNP NSDD 家兔 

分 类 号：S829.1[农业科学—畜牧学]