猪链球菌2型对细胞粘附作用及粘附因子转录水平分析  

Adherence of Streptococcus suis Serotype 2 to Cells and Analysis of Transcriptional Level of Adhesive Factors

作　　者：汪伟[1] 何孔旺[1] 倪艳秀[1] 吕立新[1] 周俊明[1] 张雪寒[1] 

机构地区：[1]江苏省农业科学院兽医研究所、农业部兽用生物制品工程技术重点实验室、国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《西南农业学报》2013年第5期2093-2098,共6页Southwest China Journal of Agricultural Sciences

基　　金：江苏省自然科学基金面上项目(BK2009327);江苏省自然科学基金重点项目(BK2010068)国家自然科学基金项目(31072155);江苏省农业科技自主创新资金项目[CX(11)2060]

摘　　要：本研究中主要采用粘附计数和目前已发表的粘附因子,通过检测它们mRNA相对转录水平,研究了SS2粘附PK-15细胞、HEp-2细胞及PIEC的能力。结果显示,总体强毒株对细胞的粘附能力较强,无毒株对细胞没有粘附能力,但这不是绝对的,并初步在mRNA水平上阐明SS2粘附细胞能力差异的原因。这为后续研究SS2粘附机制及了解SS2感染后的致病机理奠定基础。In this trial,by adhesion counting and detecting mRNA transcriptional level of the adhesive factors which had been reported,the adhesive abilities of SS2 to PK-15,HEp-2 and PIEC cells were studied.The results showed that virulent strain could adhere to cells strongly,and avimlent strain could not adhere to cells,but that was not absolute.Also,the differences of adhesion ability in mRNA transcriptional level were elucidated.This work would help to further study the mechanisms of adhesion and the pathogenesis of SS2 infection in the future.

关 键 词：猪链球菌2型 粘附作用 粘附因子 细胞培养 荧光定量PCR 

分 类 号：S828[农业科学—畜牧学]