双价抗真菌基因对甜瓜的遗传转化  

Genetic transformation of chitinase and β-1, 3-glucanase genes in melon(Cucumis melo var.makuwa Makino)

作　　者：王学征[1] 商丽敏[1] 刘宏宇[1] 纪雪岩[1] 高鹏[1] 李文滨[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《东北农业大学学报》2013年第10期61-66,共6页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(31000917);国家西甜瓜产业技术体系项目(CARS-26-02);黑龙江省博士后基金(LBHZ07236)

摘　　要：研究利用子房注射法实现双价抗真菌基因对甜瓜自交系的遗传转化,共获得19株转基因植株,转化率为0.27%。对获得的转基因植株,进行PCR鉴定。结果表明,双价基因已整合到基因组中。在枯萎病抗性检测中发现,转基因甜瓜株系比对照植株表现出中抗的有12株。表现为中抗的转基因甜瓜幼苗接枯萎病菌后,测定几丁质酶和β-1,3-葡聚糖酶的酶活性。结果表明,转基因株系的酶活性比对照非转基因植株高5倍以上。The application of ovary injection achieved two antifungal genes on melon inbred genetic transformation and obtain 19 transgenic plants, conversion rate of 0.27%. The obtained transgenic plant, the PCR identification, the results showed that the bivalent gene had been integrated into the genome; in Fusarium wilt resistance testing found, transgenic muskmelon strains than control plants showed resistance to 12 strains. Performance of transgenic muskmelon seedling by Fusarium oxysporum, determination of chitinase and β-1, 3-glucanase enzyme activity, and the results showed that transgenic strains, enzyme activity than control non-transgenic plants more than five times.

关 键 词：薄皮甜瓜 几丁质酶基因 Β-1 3-葡聚糖酶基因 子房注射 

分 类 号：S652[农业科学—果树学]