卷丹百合AFLP反应体系的建立与优化  

Establishment and optimization of AFLP reaction system for Lilium lancifolium Thunb

作　　者：雷家军[1] 于海涛[1] 王志刚[2] 周俐宏[2] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110866 [2]辽宁省农业科学院园艺分院,沈阳110161

出　　处：《东北农业大学学报》2013年第10期122-127,F0003,共7页Journal of Northeast Agricultural University

基　　金：辽宁省教育厅基金项目(202053092)

摘　　要：以卷丹百合(Lilium lancifolium Thunb.)为试材,改良CTAB法提取DNA,通过对优化酶切、连接、预扩增、选择性扩增等试验条件筛选,初步建立适合卷丹百合的AFLP反应体系。结果表明,改良CTAB法提取的卷丹百合基因组DNA符合AFLP分析要求;最佳酶切时间为37℃酶切8 h;16℃连接过夜;预扩增产物稀释30倍为宜。利用该体系筛选出12对能获得稳定、清晰扩增图谱的引物组合,为卷丹百合遗传图谱构建及分子辅助育种奠定基础。An AFLP reaction system was established with Lilium lancifolium Thunb. by optimizing some factors including enzyme digestion, ligation, pre-amplification and selective amplification. The results showed that the genomic DNA extracted by the improved CTAB method was fit for AFLP analysis. The digestion duration should be 8 h at 37 ℃. The ligation should be overnight at 16 ℃. The products of pre-amplification should be diluted by 30 times. The clear and stable amplification patterns could be obtained from 12 pairs of primer combinations with the optimized system. This paper might provide a method involving in gene mapping and marker-assisted breeding of Lilium lancifolium Thunb.

关 键 词：卷丹百合 AFLP 反应体系 优化 

分 类 号：S682.2[农业科学—观赏园艺]