UPF0497家族成员At2g39530基因参与植物非生物胁迫应答的研究  

Arabidopsis UPF0497 family member At2g39530 is responsive to diffe- rent abiotic stresses

作　　者：柏锡[1] 刘欣[1,2] 翟红[3] 朱延明[1] 才华[1] 纪巍[1] 罗晓[3] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]黑龙江中科瑞合环保技术服务有限公司,哈尔滨150090 [3]中国科学院东北地理与农业生态研究所,哈尔滨150081

出　　处：《东北农业大学学报》2013年第10期139-144,F0003,共7页Journal of Northeast Agricultural University

基　　金：教育部高等学校博士学科点专项科研基金(20102325120002);国家自然科学基金(31171578);黑龙江省科技创新团队建设计划(2011TD005)

摘　　要：UPF0497(Uncharacteristic protein family 0497)是一个功能未知的膜蛋白家族,近期研究表明,该家族5个成员与拟南芥凯氏带的形成有关。研究对UPF0497家族成员启动子序列进行分析,发现该家族两个成员(At2g39530和At2g03700)启动子序列中具有DRE顺式作用元件,推测该蛋白家族成员可能参与植物非生物胁迫应答。选取At2g39530基因进行研究,结果表明At2g39530是一种根增强性表达并且存在于细胞核与细胞质中的蛋白,以ABA非依赖途径对非生物胁迫产生响应。这一结果为研究UPF0497家族在非生物胁迫中的作用提供参考。Abstract： UPF0497 （Uncharacteristic protein family 0497） is a membrane protein family which function is unknown. Recently research showed that five members of this family are involved in casparian strip formation. In this study, promoter sequences of UPF0497 family members were analyzed, founding that promoter sequences of two members of the family （At2939530 and At2g03700） both had DRE cis-element. We speculated that members of this family might participate in abiotic stress response. We chose At2939530 for further study. The results illuminated that At2g39530 was a root enhanced expression protein and existed in nuclei and cytoplasm, responding to salinity, drought and cold in ABA-independent pathway, these results could be useful for further functional characterization of UPF0497 family genes in abiotic stress.

关 键 词：UPF0497家族 膜蛋白 胁迫响应 根增强性表达 

分 类 号：S572.01[农业科学—烟草工业]