灰霉病菌侵染垫侵染油菜叶片的超微结构观察  

Ultrastructural observation on infection cushion of grey mould fungus penetration the leaf of oilseed rape

作　　者：曹剑波[1] 张静[2] 张蕾[3] 陈千思[4] 秦利鸿[1] 李国庆[2] 

机构地区：[1]华中农业大学科学技术发展研究院电镜平台,武汉430070 [2]华中农业大学植物科学技术学院,武汉430070 [3]四川省农业科学院植物保护研究所,成都610066 [4]中国烟草总公司郑州烟草研究院烟草基因研究中心,郑州450001

出　　处：《华中农业大学学报》2013年第6期46-50,共5页Journal of Huazhong Agricultural University

基　　金：国家公益性行业(农业)科研专项(201303025)

摘　　要：利用透射电镜和扫描电镜观察灰霉病菌灰葡萄孢(Botrytis cinerea)侵染垫侵入油菜叶片的过程。观察结果发现:灰霉病菌顶端菌丝聚集通过形成侵染垫,侵染垫分泌胶状物质降解表皮细胞的细胞壁,菌丝通过破损的表皮细胞进入叶片并在叶肉细胞间及细胞内生长;侵入的菌丝进一步降解叶肉细胞的细胞壁并在叶肉组织中生长繁殖,造成叶肉细胞的细胞质凝聚、细胞器破裂等细胞坏死症状。研究结果表明:灰霉病菌侵染垫通过降解植物细胞壁侵染植物,并且在植物组织内生长过程中也会降解细胞壁,细胞壁的降解在灰霉病菌侵染和生长过程中起着重要作用。The infection cushion of penetration the leaf of mission electron microscopy and scanning electron microscopy. oilseed rape was investigated by trans- During the initial infection process, the hypha of grey mould fungus （Botrytis cinerea ） which formed infection cushions broken down the cell wall then penetrated into the leaf tissue of oilseed rape. After invading into the leaf epidermis cells, the hypha of infection cushions multiplied between the mesophyll cells or grew in mesophyll cells through breaking the cell wall. These results will illustrate the degradation of plant cell wall plays an important role in the infection and development of grey mould fungus infection cushion and provide clues to explain pathogenicity of grey mould fungus.

关 键 词：灰霉病菌 菌丝 侵染垫 超微结构 细胞壁降解 

分 类 号：Q248[生物学—细胞生物学] S432.44[农业科学—植物病理学]