泰国进口玉米种子玉米褪绿斑驳病毒的检测  

Detection of Maizechloroticmottlevirus in maize seeds imported from Thailand

作　　者：雷屈文[1,2] 李旻[1] 丁元明[1] 王云月[2] 

机构地区：[1]云南出入境检验检疫局,昆明650228 [2]云南农业大学植物保护学院,昆明650201

出　　处：《华中农业大学学报》2013年第6期51-54,共4页Journal of Huazhong Agricultural University

基　　金：国家质检总局科技计划项目(2011IK179)

摘　　要：采用DAS-ELISA对从泰国进口的6个玉米品种的种子进行玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)检测,结果显示其中1个样品为MCMV阳性,其他品种为阴性。RT-PCR检测结果进一步证实该样品为阳性。对PCR产物进行测序,将分离物序列与已发布的23条MCMV序列进行比对和系统进化分析,结果表明该分离物的序列与中国分离物和泰国分离物的遗传距离较近,同分化在进化树的同一个簇。取5号样品的100粒种子进行DAS-ELISA检测,结果仅有2粒种子为阳性,即MCMV的检出率为2%。Maize seeds imported from Thailand were tested for Maize chlorotic mottle virus （MC- MV） with DAS-ELISA. Among 6 varieties, Sample 5 was positive and others were negative. The result of RT-PCR also showed that Sample 5 was positive. The sequence of the RT-PCR product was deter- mined. This sequence was aligned with 23 sequences of other MCMV isolates and a phylogenetic analy- sis was completed. The result illustrated that this isolate had short genetic distance to Chinese or Thai i- solates and clustered in the same group. 100 seeds of sample 5 were detected for MCMV with DAS- ELISA method and 2 seeds were positive, so the detection rate of MCMV was 2%.

关 键 词：玉米种子 玉米褪绿斑驳病毒(MCMV) 序列分析 检测 

分 类 号：S41-31[农业科学—植物保护] S432.41