应用巢氏PCR对成人社区获得性肺炎患者咽拭子中肺炎支原体DNA的检测及耐药分析  

Study on the significance of detection of DNA by nest PCR and drug susceptibility of Mycoplasma pneumoniae in nasopharyngeal swabs collected from adult patients with community acquired pneumonia

作　　者：徐婉珍[1] 肖红丽[1] 阴赪宏[1] 

机构地区：[1]首都医科大学附属北京友谊医院科研办公室,北京100050

出　　处：《临床和实验医学杂志》2013年第20期1614-1616,共3页Journal of Clinical and Experimental Medicine

基　　金：北京市科技计划课题(Z101107050210018);首都医学发展科研基金(2009-1033);北京友谊医院启动课题(2010-10)

摘　　要：目的了解肺炎支原体(MP)在成人社区获得性肺炎(CAP)患者中的感染情况与成人MP耐大环内脂类抗生素的分子机制。方法收集北京友谊医院成人CAP住院患者咽拭子标本,巢氏PCR扩增红霉素作用靶位23S rRNA基因,电泳检测阳性标本行双脱氧末端终止法全自动DNA测序,测序结果与NCBI已登录的MP标准株23S rRNA基因作比对,分析差异基因。结果共收集成人CAP住院病例97例,其中咽拭子阳性25例,阳性率25.8%。15例为耐药株,耐药率为60.0%,均出现2 063位A-G的点突变。结论北京地区成人MP存在耐药现象,主要对大环内酯类抗菌药物耐药,耐药分子机制与国内外文献报道基本相似。Objective To explore the infection and the molecular mechanism of macrolide - resistance of Mycoplasma pneumoniae in a dult patients with community acquired pneumonia （CAP）. Methods The nasopharyngeal swabs from inpatients with CAP were detected for 23S rRNA, which is the target of erythromycin, by using nest PCR. Positive ones in gel electrophoresis were sequenced and compared with 23S rRNA of FH which has been published in NCBI. Results Ninety seven inpatients including 50 men and 47 women with average age of 68.69 were en rolled. Among them, 25 swabs were positive with positive rate 0f25.8%, 15 of these 25 obtained A to G transition at 2 063, showing resistance to macrolide ; and the resistant rate was 60.0%. Conclusion There is resistance to macrolide in Myeoplasma pneumoniae in adults in Beijing, its mechanism is basically similar to those results which have been published abroad and in this country.

关 键 词：社区获得性肺炎 肺炎支原体 耐药 巢氏PCR 

分 类 号：R563.1[医药卫生—呼吸系统]