蜱传脑炎病毒对人单核细胞的致病性  

Pathogenicity of tick-borne encephalitis virus to monocytes

作　　者：魏婧靖[1,2] 康晓平[2] 李裕昌[2] 吴晓燕[2] 张雨[2] 杨银辉[2] 

机构地区：[1]解放军总医院,北京100853 [2]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《微生物学报》2013年第11期1221-1225,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金(81171663);国家传染病重大专项(2013zx10004606)~~

摘　　要：【目的】确定蜱传脑炎病毒(Tick-born encephalitis virus,TBEV)对人单核细胞的感染性及对其复制增殖的影响。【方法】用蜱传脑炎病毒感染单核细胞THP-1,观察细胞病变情况。取不同时间点的细胞培养上清,测定病毒滴度,并用Real Time RT-PCR方法检测病毒核酸;用流式细胞法检测细胞感染率,以确定TBEV在THP-1细胞中的复制增殖情况;同时进行细胞活力检测,以确定TBEV感染后THP-1细胞的变化。【结果】TBEV病毒感染THP-1细胞后,可进行复制增殖,流式细胞法可检测到细胞内的病毒,感染病毒后的单核细胞活力显著降低。【结论】TBEV可在单核细胞THP-1中复制增殖,并可造成细胞活力的显著降低,提示单核细胞可能在TBEV感染机体并扩散至各组织器官过程中发挥了重要作用。[ Objective] To investigate the pathogenicity of tick-borne encephalitis virus （TBEV） to monocytes and its proliferation characteristics. [ Methods] After being infected with TBEV, the cytopathic effect of monocyte cell line THP- 1 was observed and viral titers were evaluated. Cell culture supernatants at different time points after infection were collected and the nucleic acids of TBE virus and the infection percentage were tested by using Real-time RT-PCR and fluorescence-activated cell sorting （FACS）; the survival rate of THP-1 after TBEV infection was detected by Dimethyhhiazol-carboxymethoxyp- henyl-Sulfophenyl-2H-tetrazolium inner salt （MTS）. [ Results ] Real-time RT-PCR and cytopathic assay both demonstrated that TBE virus could replicate in monocytes. The virus particles could be detected and visualized by FACS. In addition, monocyte viability was significantly decreased 5 days after infection with TEBV. [Conclusion] TBEV can efficiently replicate and proliferate inTHP-I cells, indicating that monocytes may play an important role in the process of TBEV spreading to various tissues and organs after infection.

关 键 词：蜱传脑炎病毒 单核细胞 致病性 

分 类 号：R512.3[医药卫生—内科学]