甘蓝型油菜BnDGAT1基因表达的特异性分析  

Specific Expression of BnDGAT1 Gene in Brassica napus

作　　者：马燕斌[1,2] 吴霞[1] 王霞[1,3] 杜春芳[1] 孙璇[1] 王新胜[1] 范建春[1] 李燕娥[1] 

机构地区：[1]山西省农业科学院棉花研究所,山西运城044000 [2]农业部黄土高原作物基因资源与种质创制重点实验室,太原030001 [3]山西运城学院生命科学系,山西运城044000

出　　处：《西北植物学报》2013年第10期1958-1963,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31201253);转基因生物新品种培育重大专项(2011ZX08010-003);山西省农业科学院博士基金(YBSJJ1204);山西省科技攻关项目(20120311003-2);山西省自然科学青年基金(2013021024-3)

摘　　要：该研究从甘蓝型油菜中克隆获得了二酰甘油酰基转移酶基因(DGAT),命名为BnDGAT1,并对该基因编码的氨基酸序列、蛋白结构域和系统进化树进行分析。结果表明:该基因编码的氨基酸序列包含二酰甘油酰基转移酶等多个功能结构域,并具有8个疏水跨膜结构区。系统进化分析表明,BnDGAT1与芥菜、拟南芥、旱金莲中DGAT1系统进化关系相对较近。利用定量PCR对BnDGAT1基因的RNA转录表达分析表明,在不同组织和角果的不同发育阶段,BnDGAT1基因的表达具有组织特异性,且在角果不同发育阶段,其RNA转录水平随着角果发育的成熟表达明显下调。In this paper,we isolated the diacylglycerol acyltransferase （DGAT） from Brassica napus and named BnDGAT1 gene.The amino acid sequence,protein domains and phylogenetic tree were analyzed.The BnDGAT1 protein was predicted that the amino sequences were composed of several completely functional domains,and eight hydrophobic transmembrane structures were detected by SOSUI.Phylogenetic tree analysis indicated that BnDGAT1 were closer with BjDGAT1,AtDGAT1 and TmDGAT1 than with other plant.In addition,expression profiles of BnDGAT1 were respectively analyzed using real-time PCR assays for different organization tissues and development stages of rape pod.Tissue-specific expression showed that the transcription expression levels in leaf and flower were higher than that in root and stem.Moreover,the expression levels of RNA transcription of pod were gradually down-regulated at different developmental stages of pod maturation.

关 键 词：油菜 二酰甘油酰基转移酶 表达分析 

分 类 号：Q786[生物学—分子生物学]