低温胁迫对极度濒危植物中华水韭生理特性的影响  

Effects of Low Temperature Stress on Physiological Characters of Critically Endangered Isoetes sinensis Palmer

作　　者：刘婷婷[1] 孙昊[1] 关旸[1] 刘保东[1] 

机构地区：[1]哈尔滨师范大学黑龙江省普通高等学校植物生物学重点实验室,哈尔滨150025

出　　处：《西北植物学报》2013年第10期2031-2036,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31170294)

摘　　要：以人工繁殖的极度濒危级（CR）植物中华水韭（Isoetes sinensis Palmer）为试验材料,研究了0 ℃低温环境下胁迫0~20 d,沉水叶片中保护酶系统（POD、SOD、CAT）、渗透调节物质（可溶性糖和可溶性蛋白）、叶绿素以及丙二醛（MDA）和相对电导率的变化。结果显示：随着低温（0 ℃）胁迫时间的延长,中华水韭沉水叶片的相对电导率、可溶性糖含量、CAT活性先升高后下降,SOD活性呈逐渐上升的趋势,POD活性、可溶性蛋白含量、叶绿素含量下降,MDA含量一直低于CK,且在一定范围内波动。研究表明,中华水韭沉水叶片在0 ℃低温胁迫中受到一定程度的伤害,但能通过调节自身的膜透性、渗透调节物质含量和保护酶活性系统来减轻低温伤害,维持其正常生理代谢功能,从而表现出一定的抗寒潜力。At 0 ℃ low temperature stress,the dynamic process of protective enzyme system （POD,SOD and CAT）,osmotic regulation substances （soluble sugar and soluble protein） and malondiadehyde （MDA） as well as relative electric conductivity were determined in Isoetes sinensis Palmer submerged leaves.The results showed that the relative electric conductivity,soluble sugar and activity of CAT increased at the beginning then decreased.The SOD activity gradually increased with the process of low temperature stress.The activity of POD,soluble protein and chlorophyll content decreased in the whole process of low temperature stress.MDA content were lower than the control group during the whole low temperature stress process and fluctuated within a certain range.We can concluded that the I.sinensis Palmer submerged leaves were negatively influenced by 0 ℃ low temperature stress,but the osmotic regulation substances,protective enzyme system,plasma membrane permeability could adjust themselves to avoid low temperature harms,which led to some tolerant ability of I.sinensis Palmer to the low temperature condition.

关 键 词：中华水韭 0℃胁迫 生理指标 

分 类 号：Q945.78[生物学—植物学]