结核分枝杆菌rESAT6-CFP10和rPstS1-HspX融合蛋白的血清学诊断价值  

The serodiagnostic value of recombinant fusion protein rESAT6-CFP10 and rPstS1-HspX from Mycobacterium tuberculosis

作　　者：李召东[1] 宋广忠[2] 赵爱桔 徐美华[1] 张艳[1] 范大鹏[1] 

机构地区：[1]杭州市红十字会医院检验科,310003 [2]浙江省医学科学院寄生虫病研究所,杭州310013 [3]浙江省医药经济发展中心,杭州310012

出　　处：《国际流行病学传染病学杂志》2013年第5期311-313,共3页International Journal of Epidemiology and Infectious Disease

基　　金：杭州市科技局科技计划项目（20110733Q07）

摘　　要：目的 探讨结核分枝杆菌rESAT6-CFP10（rEC）和rPstS1-HspX（rPH）融合蛋白用于结核病血清学诊断的价值.方法 以rEC和rPH融合蛋白为抗原,用金标免疫渗滤法（DIGFA）检测370份血清中的抗结核抗体,其中肺结核患者血清131份,非结核肺病患者血清114份,健康人群血清125份.结果 rEC和rPH融合蛋白单一或联合检测肺结核患者血清抗结核抗体的的灵敏度分别为70.23％、71.76％和84.73％.联合检测的灵敏度显著高于rEC或rPH单一检测,且差异有统计学意义（x2=1.181,P＜0.01 ;x2=0.036,P＜0.05）.3种方法用于检测非结核肺病患者和健康人血清的特异性差异均无统计学意义（x2=0.527、0.097,P＞0.05）.结论 rEC和rPH融合蛋白均有较高的灵敏度和特异性,联合检测有助于进一步提高结核病血清学诊断的灵敏度.Objective To evaluate the serodiagnostic value of recombinant fusion protein rESAT6-CFP10 （rEC） and rPstS1-HspX （rPH） from Mycobacterium tuberculosis.Methods The rEC and rPH were used as antigens to detect tuberculosis antibodies in serum samples from the patients with pulmonary tuberclosis （n=131）,patients with non-tuberculosis lung diseases （n=114） and healthy individuals （n=125） by dot immunogold filtration assay （DIGFA）.Results The sensitivities of rEC,rPH and combination antigens （rEC and rPH） were 70.23%,71.76% and 84.73%,respectively.The sensitivities of combination antigens was significantly higher than the rEC or rPH alone （x2=1.181,P〈0.01; x2=0.036,P〈0.05）.There were no significant differences in specificities among three methods for the serum dection of non-tuberculosis lung disease petients and healthy individuals （x2=0.527,0.097,P〉0.05）.Conclusions The rEC and rPH have high sensitivities and specificities in serodiagnosis of tuberculosis and combination detection can improve the sensitivity.

关 键 词：分枝杆菌 结核 血清学 rESAT6-CFP10 rPstS1-HspX 

分 类 号：R446.6[医药卫生—诊断学]