适用于荧光成像的化学发色团与生物正交反应研究进展  

Recent progress on the development of probes for fluorescent imaging and bio-orthogonal reactions

作　　者：张艳[1] 陈鹏[2] 姚祝军[1] 

机构地区：[1]南京大学化学化工学院,南京210093 [2]北京大学化学与分子工程学院,北京100871

出　　处：《科学通报》2013年第28期2872-2885,共14页Chinese Science Bulletin

基　　金：国家重点基础研究发展计划(2010CB833202);国家自然科学基金(91213303)资助

摘　　要：荧光成像是一种融合多门现代学科的显像方法,其运用荧光成像探针实现对细胞及动物体内的生物大分子的动态、可视化观察,为疾病的诊断与治疗提供了有力的方法.理想的荧光发色团和细胞环境友好的生物正交反应是构建性能优良的活细胞荧光成像探针的两大因素,本综述总结了近年来在荧光发色团及生物正交反应方面最新的研究进展.Fluorescent imaging is a multidisciplinary integrated technology for bio-imaging. Application of fluorescent probes allows dynamic visualization of large bio-molecules inside cells and animals, which can benefit the diagnosis and treatment of various diseases. Satisfactory fluorophores and bio-orthogonal reactions play crucial roles in the construction of ideal fluorescent probes for in vivo imaging of cellular biopolymers and biological events. This review summarized the recent advance in these two areas with examples illustrating the applications in fluorescent bio-imaging.

关 键 词：荧光成像 生物大分子 荧光发色团 生物正交反应 定量生物学 

分 类 号：O644.1[理学—物理化学]