响应面法优化黑曲霉产葡萄糖氧化酶的发酵条件  

Optimization of Fermentation Conditions for Glucose Oxidase Production by Aspergillus niger using Response Surface Method

作　　者：李瑞瑞 刘佃磊 杨晴 郝琼 姜凯凯 李丕武 

机构地区：[1]齐鲁工业大学食品与生物工程学院,济南250353 [2]山东省微生物工程重点试验室,济南250353

出　　处：《中国生物工程杂志》2013年第10期111-116,共6页China Biotechnology

基　　金：国家"863"计划资助项目(2012AA021504)

摘　　要：运用响应面法对黑曲霉(Aspergillus niger)产葡萄糖氧化酶发酵条件进行了优化。根据单因素试验结果,利用Plackett-Burman进行两因素两水平设计对影响其产酶因素进行评估并筛选出具显著效应的3种因素:葡萄糖、MgSO4·7H2O和转速。用最陡爬坡试验逼近以上三种因素的最大响应面区域后,采用Box-Behnken进行三因素三水平的设计以及响应面分析,获得最佳产葡萄糖氧化酶的培养基和培养条件组成。结果表明,发酵黑曲霉的最佳产酶条件为:葡萄糖241g/L,NaNO33g/L,K2HPO41g/L,FeSO40.01g/L,KCl 0.5 g/L,MgSO4·7H2O 0.36g/L,转速168 r/min,温度30℃。在此条件下进行产酶重复试验5次,酶活力为227.93U/g,比优化前提高了95.9%。To obtain the best producing glucose oxidase medium and culture conditions,three key factors influencing the glucose oxidase production： glucose, MgSO4·7H2O and speed, were determined by Plackett-Burman design experiment. And then response surface methods were applied. Results showed that the optimum conditions for the fermentation by Aspergillus niger were： glucose 241g/L, NaNO33g/L, K2HPO41g/L, FeSO40.01g/L, KCl 0.5 g/L,MgSO4·7H2O 0.36g/L and speed 168 r/min, under which the enzyme activity was 227.93U/g,increased 95.9 percent.

关 键 词：黑曲霉 葡萄糖氧化酶 PLACKETT-BURMAN设计 响应面法 

分 类 号：Q819[生物学—生物工程]