雌激素对小鼠骨中原癌基因c-myc蛋白的影响  

Effect of estrogen on the expression of c-myc protein of bone in mice

作　　者：李灵芝[1] 张永亮[1] 马丽英[2] 刘建中 陈虹[1] 

机构地区：[1]武警医学院药学教研室,天津300162 [2]新疆医学院

出　　处：《卫生研究》2000年第6期402-404,共3页Journal of Hygiene Research

摘　　要：为初步探讨雌激素对骨的作用机制 ,以体外培养的小鼠胚胎长骨作为模型 ,通过免疫组织化学方法检测雌酚酮对骺板静止区、增殖区和肥大区 c- myc蛋白表达的影响。结果显示 ,培养介质中雌酚酮浓度为10 - 9mol/ L 时 ,增殖区 c- m yc蛋白阳性细胞面积与对照组相比显著增加 ;浓度为 10 - 8mol/ L 时 ,除增殖区外 ,静止区阳性细胞面积也显著增加 ;当浓度增至 10 - 7mol/ L 时 ,3个区阳性细胞面积均显著增加。提示雌激素可通过促进长骨 c-In order to study the mechanism by which estrogen effects on bone, the effect of estrone on expression of c myc protein in the epiphyseal plate of fetal mouse long bone was detected by immunohistochemistry procedure in vitro. The result showed that the concentrations of estrone areas of positively staining cells increased significantly in proliferative zone at 10 -9 mol/L, in proliferative zone and resting zone at 10 -8 mol/L, and in resting zone, proliferative zone and hypertrophic zone at 10 -7 mol/L respectively. It was considered that estrogen might enforce endochondral bone formation by enhancing expression of c myc in long bone.

关 键 词：雌酚酮 长骨 雌激素 原癌基因 C-MYC蛋白 

分 类 号：R977.1[医药卫生—药品]