糖尿病足感染中耐甲氧西林葡萄球菌的脉冲场凝胶电泳分型研究  

Pulsed-field gel electrophoresis typing of the methicillin-resistant staphylococci isolated from diabetic foot infections

作　　者：丁群[1] 王鹏华[1] 褚月颉[1] 冯书红[1] 孟树优[1] 孙茜[1] 李代清[1] 

机构地区：[1]天津医科大学代谢病医院糖尿病足科卫生部激素与发育重点实验室,300070

出　　处：《中华糖尿病杂志》2013年第10期610-613,共4页CHINESE JOURNAL OF DIABETES MELLITUS

摘　　要：目的研究分离白糖尿病足感染溃疡创面的耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林表皮葡萄球菌（MRSE）的分子流行病学特点。方法收集2008年1月至2010年6月从天津医科大学代谢病医院388例糖尿病足溃疡住院患者中分离的24株MRSA和18株MRSE菌株，应用脉冲场凝胶电泳方法对菌株进行同源性分析。结果MRSA菌株相似系数为42％～97％，分为11型（A—K），12株为A型克隆株（其中11株分离于2008年的1至5月），包含12个亚型；B型2株；c型2株；其余8型各1株。MRSE菌株相似系数为34％～100％，分14型（A—N），5株A型克隆株中3株A1亚型，A2、A3亚型各1株；其余13型各1株。结论本院糖尿病足病科2008年1至5月分离的MRSA菌株很可能有A型MRSA克隆株的暴发流行，2008年6月至2010年6月呈散发感染；2008年1月至2010年6月分离的MRSE菌株呈散发性，未发现有克隆株暴发流行。Objective To study the characteristic of the molecular epidemiology of methieilfin- resistant Staphylococcus aureus （MRSA） and methieillin-resistant Staphylococcus epidermidis （MRSE） isolated from diabetic foot ulcers. Methods Total of 24 MRSA and 18 MRSE strains were isolated from 388 diabetic-foot patients hospitalized from January 2008 to June 2010 in the Tianjin Metabolic Diseases Hospital. Pulsed-fleld gel eleetrophoresis （PFCE） was used to analyze the homology of the isolates. Results The similarities of MRSA was 42%-97%. The MRSA isolates were classified into 11 PFGE types （A-K） , 12 were type A （11 of them were isolated from January to May in 2008） , 2 strains were type B, 2 strains were type C and the other 8 types only found in one strain. The similarities of MRSE was 34% -100% and 14 PFGE types （A-N） were identified. Among MRSE strains, 5 were type A （including 3 type A1, 1 type A2, 1 type A3 ） , and the other 13 types found only in one strain. Conclusion An outbreak of type A MRSA probably happened from January to May in 2008. No outbreak evidences have been identified from June 2008 to June 2010. Sporadic MRSE was found from January 2008 to June 2010 and no outbreak occurred.

关 键 词：糖尿病足 感染 耐甲氧西林金黄色葡萄球菌 耐甲氧西林表皮葡萄球菌 脉冲场凝胶电泳 

分 类 号：R587.2[医药卫生—内分泌]