乳香提取物对HL60细胞端粒酶活性影响的研究  

Affection of Boswellia Carterii Birdw on Telomerase Activityexpression in HL60 Cells

作　　者：齐振华[1] 谭柏林[1] 彭旭阳 张国平 

机构地区：[1]湖南医科大学湘雅医院血液科,湖南长沙410008

出　　处：《湖南中医学院学报》2000年第3期29-31,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省科委重点课题!(0 1 95 1 2 8 1) ;卫生部资助!课题 (94 2 0 76 )。

摘　　要：为研究乳香提取物对HL6 0细胞端粒酶活性影响 ,应用端粒酶重复序列扩增技术及聚丙烯胺凝胶电泳对HL6 0细胞端粒酶活性的表达进行检测 ,并应用乳香提取物及全反式维甲酸下调HL6 0细胞端粒酶活性。结果乳香提取物能降低HL6 0细胞端粒酶活性。其降低HL6 0细胞端粒酶活性作用与全反式维甲酸相似。To investigate the regulatary effects of Boswellia Caterii Birdw(BCB)on telomerase activity expression in leukemic cells line HL60 cells.Felomerase activity of HL60 cells was detected using talomeric repeat amplificaion protocol(TRAP)and DNA lectrophoresis assay.Results:Both BCB and All-trans retinoic acid(ATRA)could down-regulat telomerese activity of HL60 cells.BCB had the time and concentration dependent down-regulat relomerese activity of HL60 cells.Conxluaion BCB could down-regulat telomerese activity of HL60 cells and the effect of BCB on telomerase activity in HL60 cells was similar to that of ATRA.

关 键 词：乳香提取物 端粒酶活性 HL60细胞 白血病 

分 类 号：R285.5[医药卫生—中药学] R273.37[医药卫生—中医学]