新生SD大鼠大脑皮层神经元与星形胶质细胞的混合培养  

Primary co-culture of cortical neurons and astrocytes of new-born SD rats

作　　者：王承娜[1] 林丽[1] 段振芳[1] 钟飞[1] 左代英[1] 吴英良[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《药学学报》2013年第11期1729-1732,共4页Acta Pharmaceutica Sinica

基　　金：辽宁省教育厅科学研究一般项目(L2012367)

摘　　要：胶质细胞参与神经元发育的整个过程，对神经元有营养和保护作用[1]，神经元一胶质细胞之间的相互作用对中枢神经系统的功能起着重要作用[2]。然而，神经元一胶质细胞在体内相互作用的研究仍然具有一定的难度[3]，而体外实验大部分采取神经元单独培养的方法[4]，对神经元一胶质细胞之间的相互作用研究较少。This study is to establish a simple and practical co-culture method of cortical neurons and astrocytes of rats. The cortex of the new-born SD rats was digested by 0.125% pancreatic enzyme, and the differential adherence was applied to obtain the mixed cell suspension of neurons and astrocytes. A low concentration of cytarabine was used to inhibit the astrocytes in a moderate way to get neuronal and astrocyte co-culture. The morphological characteristics of the cells in different times were observed under the inverted microscope. The cells began to adhere the wall 2 h after the inoculation. Neurons and astrocytes grew in a good condition under the inverted microscope 9 days after the inoculation. The results of the immunofluorescence staining and Rosenfeld＇s staining indicated that the co-culture of neurons and astrocytes was successful and the ratio of neurons and astrocytes was close to 1 ： 1. A new neurons and astrocytes co-culture method, which is simple and convenient, was successfully established. It will be an efficient method for the related researches about neuronal and astrocyte co-culture in vitro.

关 键 词：神经元 星形胶质细胞 混合培养 新生大鼠 

分 类 号：R965[医药卫生—药理学]