乙型肝炎病毒相关性肾炎的尿液蛋白质谱研究  

Urine proteomics of hepatitis B virus associated-glomerulonephritis

作　　者：王媛[1] 刘春艳[1] 赵久阳[1] 

机构地区：[1]大连医科大学附属第二医院肾内科,116023

出　　处：《中华生物医学工程杂志》2013年第4期300-305,共6页Chinese Journal of Biomedical Engineering

摘　　要：目的研究乙型肝炎病毒相关性肾炎（HBV-GN）患者的尿液蛋白质谱特点，筛选差异表达的蛋白质。方法用丙酮沉淀法提取HBV—GN患者（n=7）、原发性膜性肾病伴慢性乙型肝炎患者（n=5）、原发性膜性肾病患者（n=7）、慢性乙型肝炎无肾脏损害患者（n=7）及健康受试者（n=6）尿液标本中的蛋白质，酶解后行高效液相色谱．电喷雾离子阱串联质谱（HPLC-ESI-IT-MS／MS）检测。得到的肽序列结果用MASCOT软件行数据库检索，用查得的蛋白序列号在Uniprot数据库中进行检索，查询相关的蛋白质的信息。5组数据用手工方法进行组间对比，分析HBV-GN患者尿液中差异表达的蛋白质。结果HBV—GN患者的尿液中检测出4种具有差异性表达的蛋白质，分别是a1抗胰凝乳蛋白酶、IgY2链c区、前列腺素D2合酶、未命名蛋白（C9JAM6）。结论a1抗胰凝乳蛋白酶、前列腺素D2合酶有可能成为HBV—GN的生物标志物。Objective To analyze the characteristics of urine proteomics in patients with hepatitis B virus associated glomerulonephritis （HBV- GN） for screening the differentially expressed proteins. Methods The urinary proteins were precipitated with acetone in 7 cases with HBV-GN, 5 with primary membranous nephropathy complicated with chronic hepatitis B, 7 with primary membranous nephropathy, 7 with chronic hepatitis B without renal damage and 6 healthy subjects. This entailed enzyme cleavage followed by high performance liquid chromatography electrospray ionization-ion trap tandem mass spectroscopy （HPLC-ESI-IT- MS/MS ） for deriving the peptide sequences that were subsequently retrieved from the database by using MASCOT software. This was followed by protein sequence searching based on the Uniprot database. The among-group difference was compared by manual calculation to analyze the differentially expressed proteins in the urine of HBV- GN patients. Results A total of 4 differentially expressed proteins, namely alpha-1-antichymotrypsin, Ig gamma- 2 chain C region, prostaglandin D2 synthase and C9JAM6 （an unnamed protein）, were identified in the urine of HBV-GN patients. Conclusion Alpha-1-antichymotrypsin and prostaglandin D2 synthase may be the potential biomarkers of HBV-GN.

关 键 词：蛋白质组学 乙型肝炎病毒相关性肾炎 生物学标记 尿分析 

分 类 号：R692.3[医药卫生—泌尿科学]