CRISP2基因在不同类型精子发生障碍中的表达及其意义  

The Expression and Significance of CRISP2 Gene in Different Types of Spermatogenic Failure

作　　者：江莉[1] 李慕军[1] 覃莉[1] 

机构地区：[1]广西医科大学第一附属医院生殖中心,530021

出　　处：《天津医药》2013年第11期1052-1054,I0003,共4页Tianjin Medical Journal

基　　金：广西高校科研资助项目(项目编号:桂教200103YB023);广西教育厅科研项目(项目编号:201010LX025);广西自然科学基金(项目编号:2013GXNSFAA019140)

摘　　要：目的探讨不同类型精子发生障碍男性精液中CRISP2 mRNA的表达水平及其意义。方法 150例男性不孕患者分为正常组、弱精子组、少精子组各50例,提取精液细胞总RNA,通过实时荧光定量聚合酶链式反应(qRT-PCR)测定3组精子中CRISP2基因的mRNA转录水平并进行比较。结果 CRISP2在正常组中的相对表达量(10.281±2.173)显著高于弱精组(2.092±0.969),差异有统计学意义(P<0.05),正常组与少精子组(9.420±2.794)差异无统计学意义(P>0.05),而弱精子组的CRISP2相对表达量也显著低于少精组(P<0.05)。结论 CRISP2在不同精子发生障碍男性中的表达显著不同,CRISP2表达的减少可能导致精子活力下降。Objective To study sperm mRNA transcript expression of CRISP2 gene in the semen of patients with different types of spermatogenetic failure. Methods A total of 150 male infertility patients were divided into normal group, asthenozoospermia group and oligozoospermia group （n=50 for each group）. Total RNA was extracted from sperm cells. Re- al-time quantitative reverse transcription polymerase chain reaction （qRT-PCR） method was used to detect CRISP2 gene mRNA levels in three groups. Results There was a significantly higher expression of CRISP2 in normal group （10.281±2.173） than that of asthenozoospermia group （2.092±0.969, P〈0.05）. There was no significant difference in the expression of CRISP2 between normal group and oligozoospermia group （9.420±2.794, P〉0.05）. The relative expression of CRISP2 was sig- nificantly lower in asthenozoospermia group than that of oligozoospermia group （P〈0.05）. Conclusion The CRISP2 expres- sion was significantly different in patients with different types of spermatogenic failure. The reduced expression of CRISP2 may lead to the decreased sperm motility.

关 键 词：精子发生 RNA RNA 信使 基因表达 CRISP2 

分 类 号：R698.2[医药卫生—泌尿科学]