TGF-β、Smad信号通路蛋白及α-SMA在糖尿病肾病患者肾组织中的表达和意义  

The Role of Expression of TGF-β and Smad Signaling Pathway and α-SMA in Patients with Diabetic Nephropathy

作　　者：房勃龙[1] 韩鸿玲[1] 张鹏[1] 崔瑾[1] 

机构地区：[1]天津医科大学总医院,300052

出　　处：《天津医药》2013年第11期1067-1069,I0002,共4页Tianjin Medical Journal

摘　　要：目的检测转化生长因子(TGF)-β、Smad信号通路蛋白和α-平滑肌肌动蛋白(α-SMA)在糖尿病肾病(DN)患者肾活检组织中的表达,探讨DN的发病机制。方法收集DN肾组织标本28例(DN组);非DM行肾切除的正常肾组织10例为对照组。应用免疫组化方法检测TGF-β1、TGF-β受体(R)Ⅰ、TGF-βRⅡ、Smad2/3、α-SMA在肾组织中的表达。结果 (1)TGF-β1、TGF-βRⅠ、TGF-βRⅡ、Smad2/3在DN组和对照组中均有表达,前者高于后者,DN组早期光镜下肾组织未见明显异常时即有明显的高表达,而随着疾病的进展未见表达增高的现象。肾小球、肾小管出现纤维化后并不表达上述因子。(2)对照组的α-SMA仅表达于肾血管、肾小球和肾小管,而肾间质未见α-SMA表达,而DN组α-SMA在上述组织中均有表达。结论TGF-β及Smad信号转导通路参与了DN的发病,DN可能与免疫复合物性肾小球肾炎具有相似的发病机制。Objective To investigate the mechanism of the pathogenesis of diabetic nephropathy （DN） by detecting the expressions of transforming growth factor （TGF）-β, Smad proteins and （x-smooth muscle actin （α-SMA） in kidney biopsy of patients with diabetic nephropathy （DN）. Methods Twenty-eight patients with DN who underwent renal biopsy were col- lected as DN group. Ten subjects without DN who underwent nephrectomy were taken as control group. The expressions of TGF-β1, TGF-β R I , TGF-β R IT , Smad2/3 and α-SMA in renal tissues were detected by immunohistochemistry stain. Results （1）TGF-β1, TGF-βR I , TGF-β R 1] , and Smad2/3 were expressed in the glomeruli and tubules of both DN group and control group, while the expressions of TGF-β and Smad proteins were significantly higher in DN group than those in control group. At the early stage of DN, TGF-β and Smad proteins were significantly expressed even though there was no remarkable lesions observed by light microscopy. There was no correlation between increased expression and the progres- sion of DN. These proteins were not expressed after glomerulus and renal tubule fibrosis. （2） In control group, α-SMA was identified only in the ~ascular walls, glomerulus and renal tubules, while it was expressed in almost all parts of kidney in DN group. Conclusion TGF-β and Smad signals involved in the pathogenesis of DN, which may have similar pathogenesis with immune complex glomerulonephritis.

关 键 词：糖尿病肾病 转化生长因子β Smad2蛋白质 Smad3蛋白质 肌动蛋白类 信号传导 活组织检查 免疫组织化学 Α-平滑肌肌动蛋白 

分 类 号：R619.6[医药卫生—外科学]