应用DiversiLab系统对肺炎克雷伯菌进行同源性分析  

The Application of DiversiLab System in Klebsiella pneumoniae Genotyping

作　　者：李静[1] 胡志东[1] 田彬[1] 徐海茹[1] 

机构地区：[1]天津医科大学总医院医学检验科,300052

出　　处：《天津医药》2013年第11期1076-1078,I0002,共4页Tianjin Medical Journal

摘　　要：目的应用DiversiLab系统对肺炎克雷伯菌进行同源性分析,为医院感染控制工作提供依据。方法对2010年天津一家甲级医院外科同一病房临床分离的8株肺炎克雷伯菌,应用以细菌基因组重复序列PCR技术(rep-PCR)为原理的DiversiLab系统分型技术进行分型,并与脉冲场凝胶电泳(PFGE)分型结果进行比较。结果药敏试验结果显示,除K8-02号菌株外,其余7株肺炎克雷伯菌对11种抗菌药物具有相同的药敏谱,且为多重耐药菌;PFGE分析显示此7株菌为同一型;DiversiLab系统同时也显示该7株菌为同一组,K8-02菌为单独一组。结论 DiversiLab系统的操作简单、快速、可重复性好、结果准确,是一种标准化和自动化系统,并且DiversiLab系统与PFGE方法可获得一致结论。,肺炎;电泳,凝胶,脉冲场;细菌分型技术;Objective To analyze Klebsiella pneumoniae by DiversiLab system, providing scientific evidence for the control of nosocomial infection. Methods Eight strains of non-duplicated clinical Klebsiella pneumoniae isolated from the surgical ward in a hospital in 2010 were typed by rep-PCR-based DiversiLab system. The results were compared with those of pulsed-field gel electrophoresis （PFGE）. Results Antimicrobial susceptibility test showed that 7 clinical strains were the same sensitivity to 11 antimierobial agents, except the strain K8-02. And these 7 clinical strains were all multi-drug re- sistant. The result of PFGE showed that 7 strains were the same pattern. The result of DiversiLab also showed that 7 strains were the same pattern, and the strain K8-02 was another pattern. Conclusion DiversiLab system is simple, quick, good re- peatability and accurate, which is a kind of standardization and automation system. DiversiLab system and PFGE method could get the same result.

关 键 词：克雷伯菌 肺炎 电泳 凝胶 脉冲场 细菌分型技术 DiversiLab系统 

分 类 号：R978.11[医药卫生—药品]