类风湿关节炎患者血清和关节液白细胞介素-17A与疾病活动的关系  

Serum and Synovial Fluid Levels of Interleukin-17A in Correlation with Disease Activity in Patients with Rheumatoid Arthritis

作　　者：林辉[1] 陈永涛[1] 周燕莉[2] 刘毅[1] 

机构地区：[1]四川大学华西医院风湿免疫科,成都610041 [2]泸州医学院风湿科

出　　处：《华西医学》2013年第10期1521-1524,共4页West China Medical Journal

基　　金：国家自然科学基金(81102274);成都市科技攻关项目(10GGYB644SF-023)~~

摘　　要：目的检测类风湿关节炎(RA)患者血清和关节液白细胞介素17A(IL-17A)的变化,探讨其与临床炎症指标、疾病活动性的关系。方法 2011年6月-2012年6月采用酶联免疫吸附试验检测30例活动性RA患者和20例健康对照血清IL-17A水平,其中18例有膝关节积液RA患者同时检测配对血清和关节液IL-17A水平。结果 RA组患者血清IL-17A水平显著高于健康对照组[(40.651±16.402)、(23.799±10.693)pg/mL,P<0.05]。RA患者关节液IL-17A水平明显高于其血清中水平[(63.555±23.405)、(43.727±17.212)pg/mL,P<0.05]。RA患者血清IL-17A水平只与疾病活动性评分(DAS28)呈正相关(r=0.498,P=0.020),而RA患者关节液IL-17A水平与DAS28和血清C反应蛋白有相关性(r=0.515,P=0.029;r=0.498,P=0.035)。结论 RA患者血清和关节液IL-17A水平与疾病活动性显著相关,提示IL-17A可作为衡量疾病活动和关节损伤的标志之一。Objective To evaluate serum and synovial levels of interleukin-17A （IL-17A） in patients with rheumatoid arthritis （RA）, and to find out the correlations between IL-17A levels and clinical inflammatory parameters and RA disease activity indices. Methods From June 2011 to June 2012, serum levels of IL-17A were detected by enzyme-linked immunosorbent assay in 30 active RA patients and 20 healthy volunteers, and paired serum and synovial fluid levels of IL-17A were measured in 18 RA patients with knee synovial fluid. Results Serum IL-17A levels were significantly higher in RA patients than those in controls [（40.651± 16.402） and （23.799 ± 10.693） pg/mL, P 〈 0. 05]. Paired synovial fluid IL-17A levels were elevated compared to paired serum in RA patients [（63.555 ± 23.405） and （43.727 ± 17.212） pg/mL, P 〈 0.05]. Serum IL-17A levels only correlated with Disease Activity Score （DAS28） （P=0.020）, while synovial fluid IL-17A levels correlated with both DAS28 and C reactive protein （r=0.515, P=0.029; r=0.498, P=0.035）. Conclusion The elevated serum and synovial IL-17A levels in RA patients parallel the degree of disease activity, which suggests the usefulness of IL-17A as a possible marker for disease activity and joint damage.

关 键 词：类风湿关节炎 血清 关节液 白细胞介素17A 

分 类 号：R593.22[医药卫生—内科学]