增殖性细胞核抗原、C-fos和Bax蛋白在人胚胎舌组织中的表达  

Expression of PCNA,C-fos and Bax proteins in human embryonic tongue tissues

作　　者：刘学红[1] 张泳[1] 

机构地区：[1]绍兴文理学院医学院组织与胚胎学教研室,浙江绍兴312000

出　　处：《浙江大学学报（医学版）》2013年第5期517-522,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省教育厅科研计划(Y201018535)

摘　　要：目的：探讨增殖性细胞核抗原（PCNA）、C-fos和Bax蛋白在人胚胎舌组织不同发育阶段的分布特征.方法：应用免疫组织化学法检测第2～4个月胎龄段共16份人胚胎舌组织内PCNA、C-fos和Bax蛋白的表达,分析其变化规律.结果：第2个月胚龄时,C-fos蛋白在人胚舌组织内未见明显阳性细胞表达分布,PCNA蛋白在人胚舌上皮组织层部分细胞和舌肌组织和纤维组织内少数细胞呈阳性表达.第3个月胎龄时,PCNA和C-fos蛋白在舌上皮组织细胞阳性表达数量最多,舌肌组织和纤维组织内有部分细胞呈阳性表达.第4个月胎龄舌肌组织和纤维组织内PCNA蛋白阳性表达数量增高,C-fos蛋白阳性表达未见明显变化.Bax蛋白仅在第3个月胎龄时呈弱阳性表达.结论：PCNA、C-fos参与调控人胚胎舌组织细胞的分化发育.Objective：To investigate of proliferating cell nuclear antigen （PCNA）,C-fos and Bax proteins in human embryonic tongue tissue of different developmental stages.Methods：Immunohistochemistry was used to detect the expressions of PCNA,C-fos and Bax proteins in embryonic tongue tissues of fetuses with 2,3 and 4 month gestational age （n =16）.One-way ANOVA and LSD-t test were employed to compare the number of positive expression cells in tongue tissues of fetuses with different gestational age.Results：In the fetuses at 2,3 and 4 months of gestation,the numbers of PCNA-positive cells in tongue epithelial tissues were 20.20 ± 7.13,39.10 ± 13.44 and 26.00 ± 9.02,respectively; those in tongue muscle and fiber tissues were 17.20 ± 8.99,22.30 ±6.57 and 32.40 ± 14.72,respectively.In fetuses at 2 month of gestation,no C-fos-positive cells were found in tongue tissues; while at 3 and 4 months of gestation,the numbers of C-fos-positive cells in the tongue epithelial layers were 25.10 ±7.91,17.40 ±2.80; those in tongue muscle and fiber tissues were 24.50 ±4.67 and 28.00 ± 7.75,respectively.Only weak positive expression of Bax protein was observed in the third month of gestation in embryonic tongue tissues.A significant difference was noted in PCNA expression in tongue epithelial layers,the muscle and fiber tissues （P ＜ 0.01 and P ＜ 0.05） among 3 embryonic periods.A significant difference was found in C-fos expression in tongue epithelial layers （P ＜ 0.01）,but not in tongue muscle and fiber tissues （P ＞ 0.05） among 3 periods.Conclusion：Dynamic changes were seen in PCNA and C-fos expressions in embryonic tongue tissues in different gestational ages of fetus,indicating these two proteins may participate in regulation of the development and differentiation of tongue tissues in human embryos and fetuses.

关 键 词：舌 胚胎 哺乳动物 增殖细胞核抗原 原癌基因蛋白质C-FOS BCL-2相关X蛋白质 免疫组织化学 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]