Efficient genome editing in plants using a CRISPR/Cas system  

作　　者：Zhengyan Feng Botao Zhang Wona Ding Xiaodong Liu Dong-Lei Yang Pengliang Wei Fengqiu Cao Shihua Zhu Feng Zhang Yanfei Mao Jian-Kang Zhu 

机构地区：[1]Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 200032, China [2]Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Seienees, Chinese Academy of Sciences, Shanghai 200032, China [3]University of Chinese Academy of Sciences, Shanghai 200032, China [4]College of Science & Technology, Ningbo University, Ningbo 315212, China [5]Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA [6]Department of Horti- culture and Landscape Architecture, Purdue University, West Lafayette, IN 47907. USA

出　　处：《Cell Research》2013年第10期1229-1232,共4页细胞研究（英文版）

摘　　要：Dear Editor, In the past few years, the development of sequence- specific DNA nucleases has progressed rapidly and such nucleases have shown their power in generating efficient targeted mutagenesis and other genome editing applica- tions. For zinc finger nucleases （ZFNs） and transcription activator-like effector nucleases （TALENs）, an engi- neered array of sequence-specific DNA binding domains are fused with the DNA nuclease Fokl [1, 2]. These nu- cleases have been successful in genome modifications by generating double strand breaks （DSBs）, which are then repaired through non-homologous end joining （NHEJ） or homologous recombination （HR） in different species, including mouse, tobacco and rice [3-5]. Recently, an- other breakthrough technology for genome editing, the CRISPR/Cas system, was developed. CRISPR （clustered regulatory interspaced short 12alindromic repeats） loci are variable short spacers separated by short repeats, which are transcribed into non-coding RNAs. The non-coding RNAs form a functional complex with CRISPR-asso- ciated （Cas） proteins and guide the complex to cleave complementary invading DNA [6]. After the initial development of a programmable CRISPR/Cas system, it has been rapidly applied to achieve efficient genome editing in human cell lines, zebrafish and mouse [7-10]. However, there is still no successful application in plants reported.

关 键 词：CAS系统 基因组 编辑 植物 DNA双链断裂 DNA结合结构域 非编码RNA DNA核酸 

分 类 号：Q78[生物学—分子生物学] TU243[建筑科学—建筑设计及理论]