活血化瘀通络中药合方及拆方对糖尿病脂肪肝大鼠SOCS-3和SREBP-1c的影响  

Effect of combined prescription and separate prescription of promoting blood circulation and removing blood stasis traditional Chinese medicine on expression of SOCS-3 and SREBP-1c in diabetic fatty liver in rats

作　　者：朱雅娜[1] 孙玉凤[1] 何莉莉[1] 任荣[1] 张玉[1] 王飞[1] 李风华[1] 张艺凡[2] 郭沛然[1] 

机构地区：[1]河北医科大学第二医院消化内科 河北省消化病重点实验室,河北省石家庄市050000 [2]河北省石家庄市中心医院中西医结合内科,河北省石家庄市050000

出　　处：《世界华人消化杂志》2013年第29期3089-3096,共8页World Chinese Journal of Digestology

摘　　要：目的:观察活血化瘀通络中药合方及拆方对糖尿病大鼠脂肪肝及细胞因子信号转导抑制物-3(suppressors of cytokine signaling protein-3,SOCS-3)mRNA和类固醇条件元件结合蛋白-1c(sterol regulatory element binding protein-1c,SREBP-1c)mRNA表达的影响,探讨其抗糖尿病性脂肪肝的作用及可能机制.方法:将50只健康♂SD大鼠随机分为正常组、模型组、中药1组(活血组,丹参、川芎)、中药2组(通络组,水蛭、地龙)和中药3组(合方组,水蛭、地龙、丹参、川芎),除正常组外,参照相关文献复制糖尿病性脂肪肝大鼠模型,造模成功后,正常组和模型组予生理盐水灌胃,中药1、2、3组分别予活血中药(丹参、川芎),通络中药(水蛭、地龙),合方中药(水蛭、地龙、丹参、川芎)灌胃,1次/d;12 wk后处死大鼠,生化法测量甘油三脂(triglyceride,TG)、胆固醇(cholesterol,TC),RT-PCR法检测肝组织SOCS-3 mRNA和SREBP-1c mRNA的表达.结果:与正常组相比,模型组及各药物干预组大鼠血清TC、TG水平明显升高(6.80 mmol/L±0.19 mmol/L,5.36 mmol/L±0.24 mmol/L,5.37 mmol/L±0.25 mmol/L,5.01 mmol/L±0.22 mmol/L vs 3.55 mmol/L±0.28 mmol/L,P<0.05)、(2.26 mmol/L±0.27 mmol/L,1.83mmol/L±0.25 mmol/L,1.82 mmol/L±0.23mmol/L,1.58 mmol/L±0.19 mmol/L vs 1.35mmol/L±0.16 mmol/L,P<0.05);与模型组相比,各药物干预组大鼠血清TC、TG水平有明显降低(5.36 mmol/L±0.24 mmol/L,5.37 mmol/L±0.25 mmol/L,5.01 mmol/L±0.22 mmol/L vs 6.80 mmol/L±0.19 mmol/L,P<0.05)、(1.83 mmol/L±0.25 mmol/L,1.82 mmol/L±0.23 mmol/L,1.58 mmol/L±0.19 mmol/L vs 2.21 mmol/L±0.21 mmol/L,P<0.05);中药各组间比较:中药3组TC、TG水平比中药1、2组低(5.01 mmol/L±0.22mmol/L vs 5.36 mmol/L±0.24 mmol/L,5.37 mmol/L±0.25 mmol/L,P<0.05)、(1.58 mmol/L±0.19 mmol/L vs 1.83 mmol/L±0.25 mmol/L,1.82 mmol/L±0.23 mmol/L,P<0.05);中药2组的TC、TG水平比中药1组低,但没有统计学意义.各药物干预组大鼠肝细胞内脂肪滴减少,脂肪囊肿消失,转为轻中度AIM： To investigate the effect of promoting blood circulation and removing blood stasis traditional Chinese medicine on the expression of suppressors of cytokine signaling protein-3 （SOCS-3） and sterol regulatory element binding protein-1c （SREBP-1c） in diabetic fatty liver in rats and to explore potential mechanisms involved. METHODS： Fifty healthy male Sprague-Dawley rats were randomly divided into five groups： a normal group, a model group, a promoting blood circulation group （salvia miltiorrhiza, rhizoma ligustici, wallichii）, a removing blood stasis group （leech, lumbricus）, and a combination group （salvia miltiorrhiza, rhizoma ligustici, wallichii, leech, lumbricus）. Diabetes was induced with streptozocin （50 mg/kg i.p.） in all the groups except for the normal group. The three treatment groups were intragastrically treated with Chinese traditional medicine which can promote blood circulate and/or remove stasis once a day for 12 wk. Triglyceride （TG） and cholesterol （TC） were measured using biochemical methods. The expression of SOCS-3 and SREBP-1c mRNAs was determined by RT-PCR. RESULTS： Compared to the normal group, the levels of TG and TC were significantly increased in the model group （6.80 mmol/L ± 0.19 mmol/L, 5.36 mmol/L ± 0.24 mmol/L, 5.37 mmol/L ± 0.25 mmol/L, 5.01 mmol/L ± 0.22 mmol/L vs 3.55 mmol/L ± 0.28 mmol/L, all P 〈 0.05; 2.26 mmol/ ± 0.27 mmol/L, 1.83 mmol/L ± 0.25 mmol/L, 1.82 mmol/L ± 0.23 mmol/L, 1.58 mmol/L ± 0.19 mmol/L vs 1.35 mmol/L ± 0.16 mmol/L, all P 〈 0.05）; however, the levels of TG and TC were significantly lower in each drug intervention group than in the model group （5.36 mmol/L ± 0.24 mmol/L, 5.37 mmol/L ± 0.25 mmol/L, 5.01 mmol/L ± 0.22 mmol/L vs 6.80 mmol/L ± 0.19 mmol/L, all P 〈 0.05; 1.83 mmol/L ± 0.25mmol/L, 1.82 mmol/L ± 0.23 mmol/L, 1.58 mmol/L ± 0.19 mmol/L vs 2.21 mmol/L ± 0.21 mmol/L, all P 〈 0.05）. The levels of TG and TC were significantly higher in the promoting blood circulation grou

关 键 词：糖尿病性脂肪肝 活血化瘀通络中药 细胞因子信号转导抑制物-3 类固醇条件元件结合蛋白-1c 

分 类 号：R259[医药卫生—中西医结合]