大网膜瓣包绕胃肠道吻合口防治糖尿病大鼠消化系吻合口漏的疗效  

Wrapping digestive tract anastomotic fistula with a pedicled gastrocolic omentum flap to prevent anastomotic leakage in diabetic rats

作　　者：潘瑞艳[1] 严庆涛[2] 曲俊生[3] 韩海林[1] 徐继马[4] 薛玲喜[1] 张义军[1] 曲梅花[1] 

机构地区：[1]潍坊医学院药学与生物科学学院药理学教研室,山东省潍坊市261053 [2]潍坊市人民医院小儿外科,山东省潍坊市261041 [3]潍坊医学院附属医院内分泌科,山东省潍坊市261041 [4]潍坊医学院附属医院消化内科,山东省潍坊市261041

出　　处：《世界华人消化杂志》2013年第29期3107-3111,共5页World Chinese Journal of Digestology

基　　金：国家教育部留学回国人员科研启动基金资助项目;No.2011年第43批;中科院核分析技术重点实验室基金资助项目;No.K1302;山东省自然科学基金资助项目;Nos.ZR2009DM024;ZR2011HM043;ZR2009CL047;山东省高等学校科技计划基金资助项目;No.J11LF33~~

摘　　要：目的:探讨大网膜瓣包绕消化系吻合口在防治糖尿病大鼠消化系吻合口漏的疗效.方法:♂Wistar大鼠30只,适应饲养环境1 wk,采用高糖高脂饮食喂养2 mo后联合腹腔注射小剂量链脲佐菌素(35 mg/kg)制备2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠模型,模型稳定7 d后分两组进行胃肠道手术,对照组(大网膜未包绕吻合口残端,n=11)和实验组(大网膜包绕吻合口,n=13).结果:对照组吻合口漏发生率为27.3%,实验组吻合口漏发生率为3.8%,与对照组相比明显降低(P=0.029),大鼠存活率显著提高.结论:大网膜瓣包绕吻合口预防胃肠道手术后吻合口漏是可行的,采用大网膜瓣包绕吻合口可显著降低糖尿病大鼠胃肠道手术吻合口漏的发生,提高大鼠存活率.AIM： To investigate the effect of wrapping digestive tract anastomotic fistula with a pedicled gastrocolic omentum flap in preventing anastomotic leakage in diabetic rats. METHODS： Experiments were performed using 6-week-old male Wistar rats （n = 30）. All animals were allowed to adapt to their environment for 1 wk （free access to food and water, 12：12-h light/dark cycle） prior to the beginning of study. Type 2 diabetes mellitus （T2DM） was induced by feeding a high-fat diet for two months and then giving a single intraperitoneal injection of low-dose 1% streptozocin （35 mg/kg） after 12 h of fasting. Seven days after model induction, the rats were divided into either a control group to receive a surgery without wrapping anastomotic fistula （n = 11） or an experimental group to receive wrapping anastomotic fistula using a pedicled gastrocolic omentum flap （n = 13）. RESULTS： The incidence of anastomotic leakage was significantly lower in the experimental group than in the control group （3.8% vs 30.8%, P = 0.029）. The survival rate was also improved in the experimental group. CONCLUSION： Wrapping digestive tract anastomotic fistula with a pedicled gastrocolic omentum flap is feasible in preventing anastomotic leakage and can significantly decrease the incidence of anastomotic leakage and improve survival in diabetic rats.

关 键 词：胃肠道吻合 糖尿病大鼠 吻合口漏 大网膜瓣 

分 类 号：R656[医药卫生—外科学] R587.1[医药卫生—临床医学]