利用环形染色体构象捕获技术对Bcl11b基因座位在细胞核内空间组织的研究  

Study on the Spatial Organization of Bcl11b Gene Locus in Cell Nucleus with the Circular Chromosome Conformation Capture Techniques

作　　者：任立成[1] 李美英[2] 孙元田[1] 苏振宇[1] 杨智[3] 李冬娜[1] 

机构地区：[1]海南医学院生物学教研室,海口571199 [2]中国热带农业科学院热带生物技术研究所,海口571101 [3]海南医学院形态学教研室,海口571199

出　　处：《中国细胞生物学学报》2013年第11期1584-1591,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31260269);海南医学院科研培育基金项目(批准号:HY2012007)资助的课题~~

摘　　要：环形染色体构象捕获(4C)技术实现了在全基因组范围内捕获与4C靶位点发生相互作用的基因座位,因而通过4C相关技术可以进一步研究靶基因座位在细胞核内的空间组织形式。该文以Bcl11b基因座位作为4C分析的靶位点,通过优化4C分析的反向巢式PCR扩增条件,实现4C分析PCR的高效扩增;并通过有限克隆筛选与普通测序分析相结合的方法,在全基因组范围内捕获到一些与Bcl11b基因座位发生潜在相互作用的基因座位。这些基因座位与靶位点间的相互作用既有发生在相同染色体内的,也有发生在不同染色体之间的。这些基因座位间的相互作用表明了Bcl11b基因座位在细胞核内复杂的空间组织形式。The genome-wide gene loci can be captured by the circular chromosome conformation capture （4C） technique, which potentially interacted with the target gene locus of the 4C assay. The spatial organization of the 4C target gene locus in cell nucleus can be further investigated using the 4C-related techniques. In this article, the Bclllb locus was choosen as the target gene locus for 4C assay. Based on the serials of optimization experiments for the PCR conditions, the nested-reverse PCR was efficiently amplified for 4C assay. Combined with the limited clone screening and the general DNA sequencing techniques, many genome-wide gene loci were captured with the 4C assay, which potentially interacted with the Bclllb gene locus. These interactions between the gene loci and the 4C target gene locus were occurred in inter-chromosome and intra-chromosome. The interactions between these gene loci showed the complex spatial organization of Bclllb locus in cell nucleus.

关 键 词：环形染色体构象捕获技术 染色体构象捕获技术 Bcl11b基因座位 染色质空间组织 

分 类 号：Q78[生物学—分子生物学]