油茶花药发育中多糖和脂滴组织化学研究  

Histochemical Study of Polysaccharides and Lipids on the Developing Anthers of Camellia oleifera Abel

作　　者：魏冬梅[1] 高超[2] 袁德义[2] 

机构地区：[1]台州学院生命科学学院,台州318000 [2]中南林业科技大学经济林育种与栽培国家林业局重点实验室,长沙410004

出　　处：《中国细胞生物学学报》2013年第11期1598-1602,共5页Chinese Journal of Cell Biology

基　　金：浙江省自然科学基金(批准号:Y3110395);国家自然科学基金(批准号:31170639)资助的课题~~

摘　　要：油茶花药发育中淀粉多糖和脂滴类物质的分布呈现一定的规律:(1)在小孢子母细胞时期,具有胼胝质壁的小孢子母细胞中有少量脂滴分布在细胞核周围,花药表皮细胞中有脂滴分布,其它药壁细胞中则没有脂滴;(2)在小孢子早期,花药表皮细胞中除了脂滴外,也出现了淀粉多糖,其他药壁细胞中没有明显变化;(3)在小孢子晚期,具有大液泡的小孢子中出现较多的淀粉多糖,花药绒毡层细胞开始退化;(4)小孢子分裂形成二胞花粉后,营养细胞中的大液泡消失,细胞中开始积累脂滴,花药壁绒毡层细胞已彻底降解,其细胞质内含物转变为较大的脂滴供二胞花粉吸收;(5)成熟时期的花粉中积累了较多的脂滴和较少的淀粉多糖作为储存物。The distribution of polysaccharides and lipids during the anther development of the Camellia oleif- era Abel displayed some characters： （1） A few lipids were accumulated in microspore mother cells, which had formed a callose wall, and no lipids appeared in other cells of the anther wall; （2） At early microspore stage, some lipids and polysaccharides were displayed in the epidermis cells, and there were no evident changes of nutrition material in other cells of the anther wall; （3） At late microspore stage, some polysacchafides appeared in the microspores, and tapetal cells began to degenerate; （4） After microspore division, with the big vacuole of vegetative cell disappearing, lipids appeared in the pollen, and the tapetal cells of anther wall completely degenerated and its cell content was transformed to some big lipids for pollen absorbing at this time; （5） The mature pollen grains of Camellia oleifera accumulated many lipids and a few ofpolysaccharides in its vegetative cell as stored materials.

关 键 词：油茶 花药发育 多糖 脂滴 

分 类 号：S794.4[农业科学—林木遗传育种]