用转染m2胆碱受体基因的CHO细胞观察知母皂甙元的作用  

The Regulation Effect of Sarsasapogenin on M_2 Receptor Density in CHO m2 Cell

作　　者：施菊[1] 胡雅儿[1] 易宁育[1] 夏宗勤[1] 

机构地区：[1]上海第二医科大学细胞调控研究室,上海200025

出　　处：《上海第二医科大学学报》2000年第6期503-505,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金!资助项目 (39570 835)

摘　　要：目的观察知母甾体皂甙元 (sarsasapogenin ,SAR)对M2 受体亚型的调节作用。　方法采用转染了m2胆碱受体基因的CHO细胞为模型 ,以非选择性的3H -QNB作单点放射配基结合分析。　结果CHOm2细胞受体密度在培养 48h达顶峰 ,以后呈进行性下降。SAR对下降的CHOm2细胞M2 受体密度有明显的上调作用 ,这一作用有一定浓度依赖性。　结论SAR对下降的M2 受体密度具有上调作用。Objective To explore the regulation effect of sarsasapogenin on M 2 receptor density in CHO m2 cell. Methods The decreased muscarinic receptor density CHO m2 cell line was used as a model. The M 2 muscarinic receptor density was measured by 3H-QNB single point radioligand binding assay. Results The time course of M 2 cholinoceptor density of CHO m2 cell showed that the M 2 receptor density reached a peak at 48h after cultured and then dropped gradually. Sarsasapogenin could significantly up-regulate the M 2 receptor density. Conclusion Sarsasapogenin can up-regulate the M 2 receptor density.

关 键 词：M2受体亚型 CHOm2细胞 知母皂甙元 放射配基分析 

分 类 号：R285.5[医药卫生—中药学]