大孔树脂纯化核桃隔膜总黄酮的工艺研究  

Purification of total flavonoids from diaphragma of Juglans regia by macroporous resin

作　　者：王国军[1] 唐辉[1] 张淑兰[2] 冯荣[1] 王幻[1] 关丽[2] 刘怡娟[1] 

机构地区：[1]石河子大学药学院,新疆石河子832000 [2]新疆生产建设兵团医院药剂科,新疆乌鲁木齐830002

出　　处：《中草药》2013年第19期2688-2692,共5页Chinese Traditional and Herbal Drugs

基　　金：兵团科技项目资助(2011BA055)

摘　　要：目的研究核桃隔膜总黄酮的大孔树脂纯化工艺。方法通过对12种不同型号大孔树脂进行静态吸附与解吸实验,优选适宜的大孔树脂,并优化分离纯化条件。结果 AB-8型大孔树脂对核桃隔膜总黄酮有较好的吸附和洗脱效果,其最佳分离纯化条件为pH 4.70,0.713 5 mg/mL的质量浓度上样,树脂的上样量为4 mL/g,上样体积流量为1.5 BV/h,依次用2 BV水洗脱,2.5 BV 50%乙醇洗脱。经AB-8树脂处理后的总黄酮质量分数达72.25%,收率为93.94%。结论 AB-8型大孔树脂用于富集核桃隔膜总黄酮效果最佳,是一种理想的分离纯化介质。Objective To investigate the purification technology of total flavonoids from the diaphragma of Juglans regia by macroporous resin. Methods Twelve macroporous resins were chosn with static and dynamic adsorption and desorption experiments to optimize the purification parameters. Results AB-8 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH value of 4.70, sample mass concentration of 0.713 5 mg/mL, the loaded amount of 4 mL/g, and loading flow rate of 1.5 BV/h. The sample was eluted by water with 2 BV and 50% ethanol of 2.5 BV, respectively. The purity of total flavonoids increased.to 72.25% after the purification, and the yield was 93.94%. Conclusion AB-8 is an ideal resin with the best enrichment for separating and purifying the total flavonoids from diaphragma of J. regia.

关 键 词：核桃隔膜 总黄酮 大孔树脂 纯化工艺 吸附动力学特性 

分 类 号：R284.2[医药卫生—中药学]