猪冠心病模型与临床病人间血清蛋白组相似性比较初探  

Primary Comparative Proteome Exploration of Similarities between Pig Heart Disease Model and Clinical Patients

作　　者：苗兰[1] 孙明谦[1] 林成仁[1] 曹进[1,2] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院基础研究中心,北京100082 [2]中国食品药品检定研究院食品化妆品检定所

出　　处：《中国药事》2013年第8期822-827,共6页Chinese Pharmaceutical Affairs

基　　金：科技部国际合作课题:中药治疗冠心病心绞痛的生物功效标志物(编号2011DFA31440)

摘　　要：目的通过蛋白组学比较研究,发现冠心病猪模型与临床病人之间的蛋白相似性,为今后发现临床相关的蛋白标记物提供基础。方法利用非标记液相色谱质谱联用测定猪与人的血清蛋白组,通过谱库搜索鉴别,确定二者之间蛋白组中相似蛋白。结果通过测定,冠心病猪模型与临床病人之间的蛋白组在疾病相关的8个功能组上存在相似性。结论冠心病猪模型与人的血清蛋白组之间存在一定的相似性,其具体与疾病相关的蛋白组差异尚待进一步挖掘。Objective Comparative between pig heart disease model and identifying protein biomarkers in proteomics research was used to discovery the proteome similarities clinical patients. The results would be provided some clues for further clinic relevant research. Methods Label free liquid chromatography-mass spectrometry method was used to identify the serum proteome of pig and patients. With the database searching, the similar proteins could be found in both sample pools. Results Through the determination, 8 functional clinical related proteome groups were found similarity between pig models and patients. Conclusion There could be found some similarity between pig heart disease models and patients. The difference of proteome on disease related need be mined in further research.

关 键 词：液质联用 蛋白组学 冠心病 猪 人 

分 类 号：R917[医药卫生—药物分析学]