HPLC法测定右旋糖酐40原料中5-羟甲基糠醛的含量  

Content Determination of 5-Hydroxyme-thylfurfural in Crude Material of Dextran 40by HPLC

作　　者：刘海静 雷琨[2] 王嫦鹤 王发 丁丽霞[3] 杜冠华[4] 

机构地区：[1]陕西省食品药品检验所,西安710061 [2]陕西中医学院 [3]中国药学会 [4]中国医学科学院北京协和医学院药物研究所

出　　处：《中国药事》2013年第9期945-948,共4页Chinese Pharmaceutical Affairs

基　　金：卫生部2009年度卫生公益性行业科研专项(编号200902008)

摘　　要：目的建立测定右旋糖酐40原料中杂质5-羟甲基糠醛含量的高效液相色谱法。方法采用十八烷基硅烷键合硅胶柱;以1.0g·L-1磷酸溶液-甲醇（90∶10）为流动相;流速为1.0mL·min-1;检测波长为284nm;柱温为（35±5）℃。结果5-羟甲基糠醛在0.10-10.00μg·mL-1质量浓度范围内与峰面积呈良好的线性关系,线性方程为Y=6.7412×104 X-1.1307×103,r=1.000;方法回收率为97.3%,RSD为0.4%。结论该法简便准确,适用于右旋糖酐40原料中5-羟甲基糠醛的含量测定。Objective To establish an HPLC method for the determination of 5-hydroxyme- thylfurfural in the crude material of Dextran 40. Methods The chromatographic conditions adopted were as follows： a C18 column was adopted with a mobile phase of 1.0g . L1 phosphoric acid solution-methanol （90 ： 10）, with the flow rate being 1.0 mL . min-1 ; the detection wavelength was 284 nm; the column temperature was maintained at （35 ± 5） ~C. Results The content of 5-hydroxyme-thylfurfural had a favorable linear relationship with the peak area within the mass concentration rage of 0.10-10.00.g . mL1, with the linear equation beingY= 6. 7412 X104 X --1.1307 ）〈103, r=l. 000; the average recovery was 97.3% and the RSD was 0.4. Conclusion The method is simple and accurate, which is suitable for the determination of 5-hydroxyme-thylfurfural in the crude material of Dextran 40.

关 键 词：高效液相色谱法 右旋糖酐40原料 5-羟甲基糠醛 

分 类 号：R927.2[医药卫生—药学]