用于提高成像灵敏度的区域DOT/FMT混合成像方法  

Improving Sensitivity of Fluorescence Molecular Tomography byRegion-Based Diffuse Optical Tomography

作　　者：武林会[1] 赵会娟[1,2] 易茜[1] 张伟[1] 高峰[1,2] 

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072 [2]天津市生物医学检测技术与仪器重点实验室,天津300072

出　　处：《光学学报》2013年第10期98-106,共9页Acta Optica Sinica

基　　金：国家自然科学基金(81371602;81271618;61108081);国家863计划(2009AA02Z413);高等学校博士点学科专项科研基金(20120032110056);天津市自然科学基金(13JCZDJC28000)

摘　　要：当前荧光分子层析(FMT)技术因假设背景光学结构均匀而导致其灵敏度、定量性和空间分辨率等主要指标与实际应用要求存在一定的差距,而基于区域标识的"粗粒度"扩散光学层析成像(区域DOT)重建算法在目标体结构先验信息的支持以及光学特性分区均匀性自然假设下能够有效获取目标体的光学结构,展开了提高FMT技术成像灵敏度的稳态测量模式下区域DOT/FMT混合成像方法的研究。数值模拟中以含有区域标记的光学数字鼠模型为背景,分别在已知数字鼠精确光学结构、利用区域DOT重建算法获取的数字鼠光学结构以及假设数字鼠各区域光学参数均匀三种背景情况下基于FMT技术重建荧光产率图像,并利用简化的仿体模型进行实验验证。结果表明,该区域DOT/FMT混合成像方法使FMT技术成像灵敏度有了显著提高,荧光产率图像具有更好的定位及量化精度。In view of low imaging resolution and quantitative accuracy in fluorescence molecular tomography （FMT） which are caused by the assumption of homogeneous optical structural background, and considering that the ＂coarse- grain＂ diffuse optical tomography （DOT） methodology based on region-labeling （region-based DOT） presents a promising tool of in vivo reconstructing background optical structure with the aid of anatomical a priori, an approach of region-based DOT guided FMT reconstruction algorithm under continuous-wave mode is developed for improving sensitivity of FMT. Numerical simulations are conducted on a region-labeled three-dimensional （3D） digital mouse atlas. The reconstructed fluorescent yield image with optical structural a priori information provided by region-based DOT algorithm is compared with the results with accurate optical structural background and hypothetical homogeneous background, respectively, to investigate the performance of this method. Physical experiments on a phantom are also conducted to assess this methodology. Our simulated and experimental reconstruction results indicate that this region-based DOT guided FMT approach can significantly improve the sensitivity of FMT, as well as its imaging resolution and quantitative accuracy.

关 键 词：成像系统 荧光分子层析成像 区域扩散光学层析成像 背景光学结构 荧光产率 

分 类 号：Q63[生物学—生物物理学]