HPLC法同时测定直缘乌头中草乌甲素及滇乌碱的含量  

Simultaneous Determination of Bulleyaconitine A and Yunaconitine in Aconitum Transsectum by HPLC

作　　者：熊耀嘉 字淑慧[2,3] 吴静[2] 熊娇[2] 吕丹[2] 李晓波[2] 杨生超[3] 沈勇[2,3] 

机构地区：[1]滇虹药业集团股份有限公司,昆明607160 [2]云南农业大学农学与生物技术学院 [3]云南农业大学云南省优势中药材规范化种植工程研究中心

出　　处：《中国药师》2013年第10期1463-1465,共3页China Pharmacist

基　　金：云南省社会发展科技计划项目(编号:2012CG007)

摘　　要：目的:建立同时测定直缘乌头中草乌甲素和滇乌碱两种C_(19)二萜生物碱类化合物含量的高效液相色谱方法。方法:采用Agilent Zorbax SB-C_(18)(250mm×4.6mm,5μm)色谱柱;流动相为0.2%三乙胺水溶液(用磷酸调节pH至3.1±0.1)-乙腈(65:35);流速为1.0ml·min^(-1);检测波长为260nm;柱温为30℃。结果:草乌甲素和滇乌碱分别在0.082~1.632μg和0.070~1.392μg范围內呈良好线性关系(r>0.9998);草乌甲素和滇乌碱的平均回收率分别为98.99%,99.49%;RSD分别为1.39%,1.24%(n=6)。结论:该方法简便、准确,分离效果好,可用于直缘乌头中草乌甲素及滇乌碱的含量测定。Objective ： To establish an HPLC method for the simultaneous determination of bulleyaconitine A （ 1 ） and yunaconitine （2） in the roots of Aconitum transsectum. Method： The two constituents were measured on an Agilent Zorbax SB-C18 （250 mm× 4.6 mm, 5 μm） column eluted with water [ containing 0.2% triethylamine, adjusting pH to （3.1 ±0.1 ） with phosphoric acid] and acetonitrile （65：35 ） at the detection wavelength of 260nm. The flow rate was kept at 1.0 ml · min^-1 and the column temperature was 30℃. Result： Linearity of each standard was established within the concentration range of 0. 082-1. 632 μg for compound 1 and 0. 070-1. 392 μg for compound 2 with the correlation coefficient above 0. 999 8. The average recovery （ n = 6 ） for compound 1 and 2 was 98.99% with RSD of 1.39% and 99.49% with RSD of 1.24% , respectively. Conclusion： The developed method is simple, ac- curate, well separated and precise, which can be used in the determination of bulleyaconitine A and yunaconitine in Aconitum transsec- turn.

关 键 词：直缘乌头 草乌甲素 滇乌碱 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]