广西地桃花水提物与抗菌药物对G^+球菌的体外联合抗菌作用  

Antibacterial Activity of Aqueous Extract of Urena Lobata L.Combined with Antibacterial Agents Against Gram-positive Bacteria in vitro

作　　者：覃巧[1] 邹小琴[1] 杨玉芳[1] 韦润莲[1] 王希斌[1] 杨莎莎[2] 黄小理[1] 刘新文[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021 [2]广西桂林医学院药学院

出　　处：《中国药师》2013年第10期1475-1478,共4页China Pharmacist

基　　金：广西壮族自治区中医药管理局项目(编号:GZZC1134)

摘　　要：目的:探讨广西地桃花水提物与抗菌药物对G^+球菌的体外联合抗菌作用。方法:采用试管二倍稀释法分别测定地桃花水提物及多种临床常用抗菌药物单药时对金黄色葡萄球菌、粪肠球菌的最小抑菌浓度(MIC),采用试管棋盘法测定地桃花水提物与抗菌药物联用对金黄色葡萄球菌和粪肠球菌的MIC,并计算联合指数(FIC)。结果:地桃花水提物分别与阿奇霉素、左氧氟沙星合用,对金黄色葡萄球菌的FIC指数分别为0.14、0.28,呈协同作用;与头孢唑林钠、克林霉素合用FIC指数分别为0.75、2.0,依次为相加作用和无关作用。地桃花水提物分别与氨苄西林钠、左氧氟沙星合用,对粪肠球菌的FIC指数分别为0.50、0.27,均为协同作用。结论:地桃花水提物与抗菌药物联用对金葡菌和粪肠球菌的MIC明显下降,呈现不同程度的抗菌效果。Objective： To investigate the inhibitory effects of aqueous extract of urena lobata L. combined with antibacterial agents against gram-positive bacteria in vitro. Method ： The minimal inhibitory concentration （MIC） of aqueous extract of urena lobata L. and the clinical antibiotics against S. aureus or E. faecalis in vitro was respectively detected by two dilution method. The MIC of aqueous extract of urena lobata L. combined with these antibacterial agents was tested by checkerboard method. The fractional inhibitory concen- tration （F1C ） was calculated. Result： The synergism （FIC = 0.14 and 0. 28, respectively） was shown in the combination of aqueous extract of urena lobata L. and azithromycin or levofloxacin against S. aureus, and the additive effect （ FIC = 0.75 ） or indifferent effect （FIC = 2. 0） was found in the combination of urena lobata L. and cefazolin sodium or clindamycin, respectively. The combination of a- queous extract of urena lobata L. with ampicillin sodium or levofloxacin both showed synergism （ FIC = 0.50 and 0.27, respectively）. Conclusion： The MICs of combination against S. aureus or E. faecalis are decreased significiantly, which shows different degree of an- tibacterial effects.

关 键 词：地桃花水提物 金黄色葡萄球菌 粪肠球菌 抗菌药物 联合抗菌 体外 

分 类 号：R285.5[医药卫生—中药学]