正交法优选乌蔹莓过氧化物酶活性的测定条件  

Optimum Conditions for Measuring Peroxidase Activity in Cayratia japonica( Thunb) Gagne by Means of Orthogonal Experiment

作　　者：黄国文[1] 

机构地区：[1]湖南科技学院生命科学和化学工程系,湖南永州425100

出　　处：《安徽农业科学》2013年第22期9228-9228,9244,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选乌蔹莓过氧化物酶活性的测定条件。[方法]以酶活力为考察指标,通过正交试验,研究酶体积、底物体积、反应体系的酸碱度和反应温度等不同条件对乌蔹莓过氧化物酶活性的影响。[结果]影响乌蔹莓过氧化物酶活性测定的因素大小顺序依次是:底物体积>温度>酶体积>pH。乌蔹莓过氧化物酶活性测定的最佳条件为:0.5 ml浓度2%的H2O2,温度为35℃,酶体积为0.1 ml,pH值为5.5。[结论]底物体积和温度是影响过氧化物酶活性的主要因素。[ Objective ] To optimize conditions for measuring peroxidase activity of Cayratia japonica（Thunb） Gagne. [ Method ] With enzyme activity as investigation index, through orthogonal test, effects of enzyme volume, substrate volume, pH value of reaction system and reaction temperature on peroxidase activity of C. japonica were studied. [ Result ] The order of influencing factors is substrate volume 〉 temperature 〉 enzyme volume 〉 pH. The optimal determining conditions are 0.5 ml 2% H202, temperature 35℃, enzyme volume 0.1 ml, pH value 5.5, [ Conclusion] The substrate concentrations and temperature are the main factors affecting the POD activity.

关 键 词：乌蔹莓[Cayratia JAPONICA (Thunb) Gagne] 过氧化物酶 活性 正交试验 

分 类 号：S567[农业科学—中草药栽培]