玉米自交系08-641不同选择方向回交改良后代的遗传变异  

The Genetic Variation of the Backcross Modified Lines Developed from the Maize Line 08-641 Selected by Different Directions

作　　者：李芦江[1] 熊尧宇[1,2] 陈文生[3] 兰海[1] 林海建[1] 杨克诚[1] 高世斌[1] 潘光堂[1] 

机构地区：[1]四川农业大学玉米研究所,农业部西南玉米生物学与遗传育种重点实验室,四川雅安625014 [2]四川省成都市新津县农村发展局,四川成都611430 [3]四川农业大学农学院,四川成都611130

出　　处：《华北农学报》2013年第5期101-105,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家玉米产业技术体系项目(CARS-02-07);"十二五"农村领域国家科技计划课题项目(2011AA10A103-2);四川省教育厅资助科研项目(13ZA0250)

摘　　要：利用SSR分子标记对玉米自交系08-641(R08)不同选择方向回交改良得到的79个BC2F4后代的遗传多样性进行分析。结果表明,利用44对具有清晰多态性的引物共检测到272个多态性等位基因位点,其中有123个位点在回交后代株系中被检测到而在轮回亲本中缺失;以种子形状和颜色不像R08为标准选择得到的回交后代株系,基因型与R08的相似程度总体低于以种子形状和颜色类似R08为标准选择得到的回交后代株,且其变异范围更广。可见不同选择方向所得回交后代选系的遗传变异存在一定的差异。因此,在回交过程中采用多向选择的策略,可能有利于提高回交改良的育种效率。In this study ,SSR molecular markers were used to analysis the genetic variation of 79 backcross modi-fied lines of the maize inbred line 08-641（R08）.The result showed that a total of 272 alleles were detected in the im-proved lines and R08,and 123 of them were detected in the modified lines but discarded in R 08.The modified line se-lected based on simialry in the shape and corlor of seeds to R 08 have lower genetic similary between R 08 than that between the lines selected based on dissimialry in the shape and corlor of seeds and R 08,and the genetic varioation of theses lines were wider .It conduvted that when the backcross breeding were used to improve the maize inbred lines , multidirectional selection based on phenotypic value were contribute to create and keep genetic variation .

关 键 词：玉米 自交系 回交 选择方向 08—641 遗传变异 SSR 

分 类 号：S153.03[农业科学—土壤学]