乳腺癌耐药蛋白BCRP高表达细胞株的构建  

Establishment of Highly Expressed Cell Strain of Breast Cancer Resistance Protein BCRP

作　　者：应帅[1] 郑婷婷[1] 陈培远[1] 许传莲[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2013年第6期877-880,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

摘　　要：BCRP属于ABC转运蛋白超家族G亚族的第二位成员,是导致临床乳腺癌治疗失败的最主要原因之一。通过构建pLJM1-bcrp重组质粒,利用病毒侵染的方法,将重组质粒转染至乳腺癌细胞株MCF-7中,通过嘌呤霉素筛选得到BCRP稳定高表达的细胞株MCF-7/BCRP,并利用半定量RT-PCR和Western-blot进行分析,分别从mRNA水平和蛋白质水平研究BCRP的表达。BCRP is the second member of ABC transport protein superfamily G subtribe and one of the main reasons causing the failure of breast cancer therapy.This paper transfects recombinant plasmid into breast cancer cell strain MCF-7 with the method of virus infection through the establishment of pLJM1-bcrp recombinant plasmid,obtains BCRP stable and highly expressed cell strain MCF-7/BCRP through puromycin screening,conducts analysis using semiquantitative RT-PCR and Western-blot and studies the expression of BCRP respectively from mRNA level and protein level.

关 键 词：BCRP ABCG2 重组表达载体 病毒侵染 

分 类 号：Q786[生物学—分子生物学]