农药氟虫腈作用下斑马鱼miR-155对UHRF1的调控作用  

Regulating Effect of Zebrafish miR-155 on UHRF1 under Action of Pesticide Fipronil

作　　者：张杰[1] 张凯[1] 李鹿丰[1] 郭江峰[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2013年第6期891-895,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省自然科学基金项目(LY12B07012);浙江省大学生科技创新活动计划项目(2012R406063)

摘　　要：农药氟虫腈对生物体具有慢性神经毒性,对生态环境具潜在的危险性。通过实验分析不同浓度氟虫腈等农药处理后,miRNA和相关靶基因在核酸和蛋白水平的表达,有助于明确miR-155和相关靶基因在外源化学物质毒理学效应中的作用与关系。本研究以dre-miR-155为研究对象,通过生物信息学分析,对其靶标进行预测,结合互补"种子"序列、结合的自由能以及各数据库中的排位等因素,选取所预测靶基因UHRF1进行研究,并利用荧光定量PCR、Western blot从基因和蛋白水平研究miR-155对基因UHRF1的调控作用。结果表明,在氟虫腈的作用下miR-155表达上调,UHRF1表达下调;UHRF1蛋白表达量随miR-155表达的增加而减少,miR-155可能对UHRF1的表达有负调控作用。miR-155对UHRF1的调控作用的研究对毒理学、生物医学等领域具有重要意义。Pesticide fipronil has chronic neurotoxicity to living body and potential risk to ecological environment.The experimental analysis on expression of nucleic acid and protein level in miRNA and related target gene treated by pesticides such as fipronil of different concentrations is helpful for determining the effect and relation of miR-155 and related target gene in toxicological effect of exogenous chemicals.With dre-miR-155 as the research object,this research predicts its drone through bioinformatics analysis,selects the predicted target gene UHRF1 for research in combination with complementary seed sequence,binding free energy and ranking in each database etc.and studies the regulating effect of miR-155 on gene UHRF1 from gene and protein level using fluorescent quantitation PCR and Western blot.The result shows miR155 expression up-regulation and UHRF1 expression down-regulation under the action of fipronil; UHRF1 protein expression quantity reduces with the increase of miR-155 expression and miR-155 might have negative regulation effect on UHRF1 expression.The research on regulating effect of miR-155 on UHRF1 has important meanings for such fields as toxicology and biomedicine.

关 键 词：氟虫腈 MIR-155 斑马鱼 UHRF1 毒理学 生物医学 

分 类 号：Q89[生物学]