药典法和新方法测定人参皂苷含量比较  

Comparative Study of Content Determination of Ginseng Pharmacopoeia Method and a New Method for the Content Determination of Ginseng

作　　者：付聪[1] 杨艳芳[2] 王业静[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065 [2]湖北中医药大学中药资源与中药复方教育部重点实验室,湖北武汉430065

出　　处：《亚太传统医药》2013年第11期25-27,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:探寻更高效的人参皂苷提取方法,提高人参皂苷检出率,简化操作。方法:以人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量为评价指标,乙醇为提取溶液,采用正交试验法,以高效液相色谱法进行含量测定,得出最佳的人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的检测方法。结果:优选的提取条件为将人参药材粉碎成中粉,以60%的乙醇浸泡提取24h。结论:乙醇浸泡提取法简化了制样步骤,减少了溶剂消耗,可以同时测定人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量,为人参的质量评价提供参考。Objective： To find for a new and more efficient sample preparation and detection method of ginseng saponin and simplify the technology. Methods：In this thesis, taking detection rate of ginsenoside Rgl, ginsenoside Re and ginsenoside Rbl as index and using orthogonal test method , HPLC was used to determinate the contents of ginsenoside Rga , ginsenoside Re and ginsenoside Rb1. The sample preparation technology before detection was optimized. Results： Granularity into medium powder before 60% ethanol extraction for 24 hours was screened as the optimum preparation technology. Conclusion ： The sample preparation steps were sim- plified by adopting ethanol immersion method. Also, solvent consumption was induced by the preparation technology. This technolo- gy for simultaneous determination of ginsenoside Rgl , ginsenoside Re and ginsenoside Rb1 provides a reference for ginseng＇s quality evaluation.

关 键 词：人参皂苷Rg  人参皂苷RE 人参皂苷RB1 正交设计 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]