SSCP检测内毒素致mtDNA基因突变及阳离子A的保护作用  

detection of endotoxin-induced mtDNA gene mutation and protection effect of CA

作　　者：刘倩[1,2] 周铭涛[1] 高洪[1] 高利波[1] 赵汝[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]赤峰学院医学院,内蒙古赤峰024000

出　　处：《中国兽医学报》2013年第11期1707-1711,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30560113);云南省高端科技人才引进计划项目(2009CI125);云南省现代农业生猪产业技术体系建设专项基金;云南省高校科技创新团队支持计划资助(云教科[2011]14号)

摘　　要：将60只健康试验大鼠随机分为3组:正常对照组(A组)、ET处理组(B组)、CA+ET处理组(C组),运用PCR-SSCP技术对肝脏mtDNA tRNACys-Tyr、COI基因片段一等基因进行了分析,同时运用分光光度计法对血清丙二醛的含量进行测定,观察ET致mtDNA的突变效应以及CA对该效应的保护作用。,结果发现,B组mtDNA tRNACys-Tyr基因片段在第5238与5242碱基处发生了插入1个碱基T的插入突变,COI一基因片段发生T5322G和T5331G处置换点突变,其他各组无基因突变发生。B组血清MDA含量均明显高于其他二组(P<0.05)、C组明显低于B组但高于A组(P<0.05)。结果表明,ET可导致mtDNA基因发生突变,而CA对ET致基因突变具有保护效应。To investigate the mutational effect of endotoxin （ET）-induced mtDNA and the protec- tive effect of cation A （CA）,sixty experimental rats were divided randomly into 3 groups：normal control group （group A）,ET treated group （group B）,and CA＋ET treated group （group C）. The liver mtDNA was extracted for detecting the mutations of tRNAcys-Tyr ,COI fragment one gene by polymerase chain reaction-single stranded conformation polymorphism （PCR-SSCP） tech- nique. The results revealed that a mutation was tRNAcys-Tyr of group B,which was inserted T be- tween 5238 and 5242 region,another mutation happened in T5322G and T5331G of the mtDNA COI one gene, no mutations of this gene was detected in group A and C. Serum MDA contents of group B were significantly higher than those of group A and C （P〈0.05） ,those of group C were significantly lower than those of group B and higher than group A （P〈0.05）. It can be concluded that ET could cause the mutation of mtDNA gene,and CA has antagonistic and protective effect on the mutation of mtDNA gene caused by ET.

关 键 词：内毒素 聚合酶链式反应单股构象多态性分析 mtDNA基因突变 阳离子A 保护效应 

分 类 号：S852.44[农业科学—基础兽医学]