辣椒愈伤组织诱导的研究  

Study on Callus Induction fromin vitro Capsicum annuum L.

作　　者：谢礼洋[1] 匡华琴[1] 赖钟雄[1] 刘生财[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《黑龙江农业科学》2013年第11期17-20,共4页Heilongjiang Agricultural Sciences

基　　金：福建农林大学本科教学改革资助项目(111412099)

摘　　要：为更好地利用辣椒愈伤组织分化再生植株及遗传转化,以2个辣椒品种的种子为试材,诱导无菌苗,取不同苗龄的胚轴作为外植体,比较它们之间愈伤组织诱导效果的差异,并探讨了不同浓度的IAA与6-BA组合对辣椒愈伤组织诱导的影响。结果表明:以辣椒苗龄为16d的试管苗作为外植体诱导愈伤组织的效果最好;辣椒胚轴诱导愈伤组织的最佳培养基为MS+0.8mg·L-1 IAA+1.0mg·L-1 6-BA+4.0mg·L-1 AgNO3,但辣椒王诱导愈伤组织效果好于日本朝天椒,说明辣椒基因型对愈伤组织诱导有重要作用。In order to use callus to regenerate plant and do genetic transformation, taking two varieties of Capsi cum annuum L. seeds as materials which were used to induce in vitro plantlets（（＇apsicurn annuum L ）, and tak ing hypocotyls of different seedling age as explants, the differences between their induction effect were corn pared,and the induction effect ofdifferent concentrations combination of IAA and 6-BA on callus were dis cussed. The results showed that the best seedling age to induce callus was at 16 d as explant callus. The best medium for callus induction by hypocotyls was MS medium supplemented with 0.8 mg＂ L. IAA, 1.0 mg. L . 6 BA and 4.0 mg .L 1 AgNO,~, and the effect of callus induction by Pepper king was better than Japanese pod pepper, it showed that genotype had highly significant impact on induction of callus from in vitro Capsicum an nuum L.

关 键 词：辣椒 愈伤组织 苗龄 激素 基因型 

分 类 号：S641.3[农业科学—蔬菜学]