Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields  

作　　者：Huang Zhu Xiaojing Cai Xianqun Fan 

机构地区：[1]Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200011, China [2]Department of Ophthalmology, Xinhua Hospital, Shanghai JiaoTong University School of Med-icine [3]Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200092, China [4]Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200011, China

出　　处：《Journal of Traditional Chinese Medicine》2013年第5期664-668,共5页中医杂志（英文版）

基　　金：Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission(10411966200);the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau(2009s023);the Shanghai Leading Academic Discipline Project(S30205)

摘　　要：OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.OBJECTIVE： To study the effect of puerarin on matrix metalloproteinase-2（MMP-2） gene and protein expression in human fetal scleral fibroblasts（HFSFs） exposed to extremely low frequency electromagnetic fields（ELF-EMF）.METHODS： Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h. The experimental groups were divided into subgroups treated with 0, 0.1, 1and 10 μM puerarin respectively. The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS： MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h. Puerarin at the concentration of 0.1 μM reversed this effect by 8.53% in mRNA and by 17.97%in protein expression（P0.05）. The effect was more prominent at higher concentrations（1 and 10 μM,P0.01）.CONCLUSION： Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells. Puerarin reversed the action to some extent in a specific concentration range. Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.

关 键 词：Puerarin Matrix metalloproteinase 2 Electromagnetic fields Sclera Fibroblast 

分 类 号：R276.7[医药卫生—中医五官科学]