多花黄精组培快繁技术初探  

The Preliminary Study on Technology of Tissue Culture and Rapid Propagation for Polygonatum cyrtonema Hua

作　　者：万学锋[1,2,3] 陈菁瑛[1,2] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]福建省农业科学院药用植物研究中心,福建福州350003 [3]福建省漳州市漳州农业科学研究所,福建漳州363005

出　　处：《中国现代中药》2013年第10期850-852,共3页Modern Chinese Medicine

基　　金：福建省科技项目"闽产根茎类药材种苗繁育技术研究"(2011R1020-2)

摘　　要：目的:初步研究福建多花黄精快速繁殖技术,方法:以多花黄精的根茎为外植体,比较不同的消毒剂和消毒时间,不同的细胞分裂素对多花黄精快繁的影响,优选出最适条件。结果:0.2%HgCl2消毒15 min最佳,污染率低存活率高;BA比其他细胞分裂素有利于多花黄精的分化,在其浓度40 mg·L-1时,增殖系数最高为2.80。To study the preliminary technology of tissue culture and rapid propagation for Polygonatum cyrtonema Hua. The rhizome of Polygonatum cyrtonema Hua was used to be explant to compare the effect of disinfectants, disinfection time, cytokinins and BA, then established a better condition. The best disinfectant was 0. 2% HgC12, disinfection time was 15minutes; BA was better than the other cytokinins, when concentration was 40 mg · L^-1 with with the multiplication coefficient of 2.80.

关 键 词：多花黄精 组培快繁 

分 类 号：R282.21[医药卫生—中药学]