基于CD14,IL-1β表达及SGC-7901细胞凋亡变化探讨清热化湿方防治胃癌的机制  

Effect of Qingre Huashi Dectction on Regulating SGC-7901 Cell Apoptosis by Mediating CD-14 and IL-1β for Prevention and Treatment of Colon Cancer

作　　者：张保静[1] 陈远能[1] 张涛[1] 

机构地区：[1]广西中医药大学,南宁530011

出　　处：《中国实验方剂学杂志》2013年第20期246-250,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学基金项目(2010GXNSFA013219);广西卫生厅重点项目(2010jjA40346)

摘　　要：目的:观察清热化湿方对人胃腺癌细胞7901增殖、凋亡及CD14基因、肿瘤坏死因子α(TNF-α)及白细胞介素1β(IL-1β)表达的影响。方法:采用血清药理学方法,对照组予胎牛血清,治疗组分别予不同体积分数(5%,10%,15%,20%,30%)清热化湿方含药血清干预体外培养的胃腺癌细胞SGC-7901,应用四甲基偶氮唑盐微量酶反应比色法(MTT法)检测细胞增殖,流式细胞仪分析细胞凋亡率、细胞周期,免疫组化、Real-time PCR技术检测CD14,TNF-α,IL-1β蛋白及mRNA表达情况。结果:与对照组比较,治疗组SGC-7901细胞存活率明显下降(P<0.05);与对照组比较,治疗组S期细胞减少、G0/G1期细胞增多,且与药物质量浓度有剂量依赖性(P<0.05)。治疗组可明显下调CD14,TNF-α,IL-1β蛋白及mRNA表达并促进细胞凋亡(P<0.05),以中剂量更为明显。结论:清热化湿方可能通过调控CD14,TNF-α,IL-1β表达,介导SGC-7901细胞S期,诱导细胞凋亡,发挥防治胃癌的效应。Objective：To investigate the intervention effect and potential mechanism of Qingre Huashi decoction （QHD） for preventing the onset of colon cancer by observing SGC-7901 cell proliferation and apoptosis along with the expression of CD14 gene,tumor necrosis factor alpha and interleukin 1 beta.Method：SGC-7901 cells were divided into control group,intervention group.In vitro,the control group was offered fetal calf serum.The intervention group was fed with serum containing QHD for 5％,10％,15％,20％,30％.The multiplication,proliferation and apoptosis of SGC-7901 cell were detected by MTT and flow cytometry respectively.The expression of CD14,TNF-α and IL-1β gene was assayed by immunohistochemistry and real-time PCR.Result：Compared with control group,the survival of SGC-7901 cell was decreased in QHD group （P ＜ O.05）.In QHD group,the cells in G0/G1 phase were increased but the S phase cell was on the decline.Those changes were correlated with the culturing content.There is a significant difference between QHD group and control group in those changes as mentioned above （P ＜ 0.05）.Especially,the effect of middle concentration serum containing QHD on inducing cell apoptosis and down regulating the expression of CD14,TNF-α,IL-1β was obvious （P ＜0.05）.Conclusion：QHD exerts a distinct effect on preventing the onset of colon cancer by mediating SGC-7901cell S time,cell composition and regulating the expression of CD14,TNF-α and IL-1β and inducing cell apoptosis.

关 键 词：清热化湿方 人胃腺癌细胞SGC-7901 CD14 肿瘤坏死因子Α 白细胞介素1Β 

分 类 号：R285.5[医药卫生—中药学]