姜黄素对TNF-α诱导大鼠星形胶质细胞MCP-1表达和释放的影响  

Effect of curcumin on tumor necrosis factor-alpha-induced expression and release of monocyte chemoattractant protein-1 in rat astrocytes

作　　者：王睿娴[1] 袁超[1] 沈露露[1] 李军[1] 曹红[1] 

机构地区：[1]温州医学院附属第二医院麻醉科温州医学院疼痛医学研究,325027

出　　处：《中华麻醉学杂志》2013年第8期916-919,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81073125）;浙江省自然科学基金（Y2090252）

摘　　要：目的 探讨姜黄素对TNF-α诱导大鼠星形胶质细胞单核细胞趋化蛋白-1（MCP-1）表达和释放的影响.方法 新生SD大鼠5只,雌雄不拘,处死后取大脑皮层进行原代星形胶质细胞培养,采用神经胶质酸性蛋白免疫荧光染色法鉴定星形胶质细胞纯度.采用随机数字表法,将细胞分为6组（n=15）：正常对照组（C组）不做任何处理；TNF-α组（T组）：加入TNF-α,终浓度20 ng/ml,孵育2h;TNF-α＋不同浓度姜黄素组（Cur5组、Cur10组、Cur20组）：加入姜黄素,终浓度分别为5、10、20μmol/L,孵育24h,弃去培养液后再加入TNF-α,终浓度20 ng/ml孵育2h；TNF-α＋溶剂对照组（D组）：加入DM-SO,终浓度1μl/rnl,孵育24h后加入TNF-α,终浓度20 ng/ml孵育2h.各组处理结束后,采用免疫组化法测定星形胶质细胞MCP-1的表达；采用ELISA方法检测MCP-1胶释放量.结果 与C组相比,其余5组星形胶质细胞MCP-1表达和释放量升高（P＜0.05）；与 T组相比,Cur20组MCP-1表达和释放量明显降低（P＜0.05）,Cur5组、Cur10组和D组星形胶质细胞MCP-1表达和释放量差异无统计学意义（P＞0.05）；与Cur5组相比,Cur20组星形胶质细胞MCP-1表达和释放量降低（P＜0.05）,Cur10组差异无统计学意义（P＞0.05）.结论 姜黄素可抑制TNF-α诱导的大鼠星形胶质细胞MCP-1表达和释放,该作用与剂量有关,该作用可能是其减轻神经病理性痛的机制之一.Objective To investigate the effect of curcumin on tumor necrosis factor-alpha （TNF-α）-in-duced expression and release of monocyte chemoattractant protein-1 （MCP-1） in rat astrocytes.Methods The primary astrocytes were prepared from the cerebral cortex of 5 neonatal Sprague-Dawley rats and cultured.The cultured cells were identified by immunofluorescence staining with glial fibrillary acid protein.The cells were then divided into 6 groups （n =15 each）：control group （group C）,TNF-α group （group T）,TNF-α＋ different concentrations of curcumin groups （Cur5,Cur10 and Cur20 groups）,and TNF-α＋ solvent control group （D group）.TNF-α with the final concentration of 20 ng/ml was added and the cells were incubated for 2h in T group.In Cur5,Cur10 and Cur20 groups,curcumin with the final concentrations of 5,10 and 20μmol/L was added,respectively,the cells were incubated for 24h and then the culture medium was abandoned,TNF-α with the final concentration of 20 ng/ml was added and the cells were then incubated for another 2h.In group D,dimethyl sulfoxide with the final concentration of 1 μl/ml was added,the cells were then incubated for 24h,then TNF-α with the final concentration of 20 ng/ml was added and the cells were incubated for another 2h.After treatment in each group,the expression of MCP-1 was determined by immunohistochemistry and the release of MCP-1 was determined by ELISA.Results Compared with group C,the expression and release of MCP-1 was significantly increased in the other five groups （P ＜ 0.05）.Compared with group T,the expression and release of MCP-1 was significantly decreased in group Cur20 （P ＜ 0.05）,and no significant changes in the expression and release of MCP-1 were found in Cur5,Cur10 and D groups （P ＞ 0.05）.Compared with group Cur5,the expression and release of MCP1 was significantly decreased in group Cur20 （P ＜ 0.05）,and no significant change in the expression and release of MCP-1 was found in group Cur10 （P ＞ 0.05）.Conclusion C

关 键 词：姜黄素 星形细胞 趋化因子CCL2 肿瘤坏死因子α 

分 类 号：R285.5[医药卫生—中药学]