不同浓度七氟醚麻醉对新生大鼠认知功能的影响  

Effects of different concentrations of sevoflurane on cognitive function in neonatal rats

作　　者：谢鹃[1] 孙建良[1] 孔飞娟[1] 周小莲[1] 罗颖[1] 

机构地区：[1]杭州市第一人民医院麻醉科,310006

出　　处：《中华麻醉学杂志》2013年第8期948-950,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价不同浓度七氟醚麻醉对新生大鼠认知功能的影响.方法 新生SD大鼠24只,7日龄,雌雄不拘,体重12～16g,采用随机数字表法,将其分为3组（n=8）：对照组（C组）和不同浓度七氟醚麻醉组（Sev12组）.C组吸入含30％氧气的空氧混合气体,Sev12组分别吸入2％、4％七氟醚6h.于吸人七氟醚后2和8周时测定认知功能（旷场实验和水迷宫实验）.结果 与C组比较,Sev1,2组新生大鼠中央格停留时间和2 min内穿越格子数差异无统计学意义（P＞0.05）,Sev1组吸入七氟醚后2周、Sev2组吸入七氟醚后2和8周新生大鼠逃避潜伏期延长,原平台穿越次数减少,第3象限活动时间缩短（P＜0.05）；与Sev1组比较,Sev2组新生大鼠中央格停留时间和2 min内穿越格子数差异无统计学意义（P＞0.05）,吸入七氟醚后2和8周逃避潜伏期延长,原平台穿越次数减少,第3象限活动时间缩短（P＜0.05）.结论 吸入2％和4％七氟醚6h麻醉均可引起新生大鼠认知功能降低,随浓度升高降低认知功能的效应增强.Objective To evaluate the effects of different concentrations of sevoflurane on the cognitive function in neonatal rats.Methods Twenty-four neonatal Sprague-Dawley rats of both sexes,aged 7 days,weighing 12-16g,were randomly divided into high concentration sevoflurane group （group Sev1）,low concentration sevoflurane group （group Sev2） or control group （group C）,with 8 rats in each group.Sev1 and Sev2 groups were exposed to 2％ and 4％ sevoflurane for 6h,respectively,and C group inhaled gas mixture composed of 30％ oxygen.At 2 and 8 weeks after exposure to sevoflurane,cognitive function was assessed using the open field test and Morris water maze test.Results In the open field test,no significant differences were observed among the three groups （P ＞ 0.05）.Morris water-maze test showed that the escape latency was significantly prolonged,and the frequency of crossing the original platform and the time spent in the third quadrant were decreased at 2 weeks after exposure to sevoflurane in Sev1 group and at 2 and 8 weeks after exposure to sevoflurane in Sev2 group as compared with group C （P ＜ 0.05）.Morris water-maze test showed that the escape latency was significantly prolonged,and the frequency of crossing the original platform and the time spent in the third quadrant were decreased at 2 and 8 weeks after exposure to sevoflurane in Sev2 group compared with Sev1 group （P ＜ 0.05）.Conclusion Exposure to 4％ and 2％ sevoflurane for 6h both can induce cognitive decline and the decreasing effect is enhanced with the increasing concentration in the neonatal rats.

关 键 词：麻醉药 吸入 婴儿 新生 认知 

分 类 号：R614[医药卫生—麻醉学]