右美托咪定对大鼠海马神经细胞氧糖缺失/复氧复糖时OGG1 mRNA表达的影响  

Effect of dexmedetomidine on expression of OGG1 mRNA in rat hippocampal neurons subjected to oxygenglucose deprivation/reoxygenation and restoration

作　　者：斯妍娜[1] 鲍红光[1] 韩流[1] 张媛[1] 蔡朦朦[1] 谢欣怡[1] 

机构地区：[1]南京医科大学附属南京医院南京市第一医院麻醉科,210006

出　　处：《中华麻醉学杂志》2013年第8期1003-1006,共4页Chinese Journal of Anesthesiology

基　　金：南京市医学科技发展项目（YKK12085）;力邦科学研究基金（CSA2012LB018）

摘　　要：目的 评价右美托咪定对大鼠海马神经细胞氧糖缺失/复氧复糖时8-羟基鸟嘌呤DNA糖苷酶（OGG1） mRNA表达的影响.方法 选取出生3d内的清洁级SD大鼠,培养原代海马神经细胞,以1×106/ml的细胞密度接种于96孔板（100μl/孔）或6孔板（2ml/孔）,采用随机数字表法,将其分为5组（n=30）：对照组（C组）、氧糖缺失/复氧复糖组（OGD/R组）、不同浓度右美托咪定组（DEX1组、DEX2组和DEX3组）.C组常规培养,其余各组建立氧糖缺失/复氧复糖模型.DEX1组、DEX2组和DEX3组于氧糖缺失前2h加入右美托咪定,终浓度分别为0.1、1.0和10.0μmol/L,于复氧复糖24h时,苏木精伊红染色后观察细胞病理学结果,采用MTT法测定细胞存活率,比色法测定细胞SOD活性和MDA含量,RT-PCR法检测细胞OGG1 mRNA表达水平.结果 OGD/R组细胞病理学损伤明显,DEX1组、DEX2组和DEX3组细胞病理学损伤程度明显减轻.与C组比较,OGD/R组、DEX1组、DEX2组和DEX3组细胞存活率降低,MDA含量升高,SOD活性和OGG1 mRNA表达水平降低（P＜0.05）.与OGD/R组比较,DEX1组、DEX2组和DEX3组细胞存活率升高,MDA含量降低,SOD活性和OGG1mRNA表达水平升高（P＜0.05）.DEX1组、DEX2组和DEX3组间上述指标比较差异无统计学意义（P＞0.05）.结论 右美托咪定可通过上调大鼠海马神经细胞氧糖缺失/复氧复糖时OGG1 mRNA表达而减轻神经细胞氧化损伤.Objective To evaluate the effect of dexmedetomidine on the expression of 8-Oxoguanine DNA glycosylase 1 （OGG1） mRNA in rat hippocampal neurons subjected to oxygen-glucose deprivation/reoxygenation and restoration （OGD/R）.Methods Hippocampal neurons isolated from pathogen-free neonatal Sprague-Dawley rats born within 3 days,were cultured primarily and seeded in 96-well plates （100 μl/well） or 6-well plates （2 ml/well） at the density of 1 × 106 cells/ml.The cells were randomly divided into 5 groups （n=30 each）：control group （group C）,group OGD/R,and different concentrations of dexmedetomidine groups （DEX1-3 groups）.The cells were cultured in normal culture medium in group C and the cells were subjected to OGD/R in the other groups.In DEX1-3 groups,dexmedetomidine with the final concentrations of 0.1,1.0 and 10.0μmol/L were added,respetively,at 2h before OGD.At 24h of restoration,hippocampal neurons were stained with haematoxylin and eosin （H.E） for examination of pathological changes,the cell survival rate was detected by MTT method,the activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） were detected by colorimetric method,and the expression of OGG1 mRNA was detected by RT-PCR.Results The pathological changes of neurons were obvious in group OGD/R,and the pathological changes of neurons were significantly mitigated in DEX1,DEX2 and DEX3 groups.Compared with group C,the cell survival rate and SOD activity were significantly decreased,MDA content was increased,and the expression of OGG1 mRNA was down-regulated in OGD/R,DEX1,DEX2 and DEX3 groups （P ＜ 0.05）.Compared with group OGD/R,the cell survival rate and SOD activity were significantly increased,MDA content was decreased,and the expression of OGG1 mRNA was up-regulated in DEX1,DEX2 and DEX3 groups （P ＜ 0.05）.There was no significant difference in the indices mentioned above between DEX1,DEX2 and DEX3 groups （P ＞ 0.05）.Conclusion Dexmedetomidine may protect hippocampal neurons a

关 键 词：右美托咪啶 DNA糖基化酶类 海马 神经元 再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]